These cryopreserved human hepatocytes are suitable for use in plated metabolism applications.
Cells per vial: 4-8 million
Prototypical cytochrome P450 substrates midazolam, tolbutamide, and dextromethorphan were used to assess the enzymatic function of CYP3A4⁄5, CYP2C9 and CYP2D6 respectively. The disappearance of parent was monitored by LC-MS⁄MS analysis and intrinsic clearance (CLint) values determined by linear regression.
Get the Results You Need with GIBCO Hepatocytes High quality hepatocytes, with high viabilities, in vivo-like enzyme expression levels, and proper cell morphology increase the ability to draw in vitro⁄in vivo correlations and make sound decisions regarding a compound's fate. Experienced technicians and proprietary isolation techniques combined with stringent release specifications ensure you receive the highest quality cryopreserved hepatocytes.
Each lot is tested for: • Phase I and II enzyme activities • Proper morphology • Viabilities generally >75% • Attachment efficiency • Monolayer formation and integrity
For research use only. Not intended for human or animal therapeutic or diagnostic use.