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Gibco™

Human Plateable Hepatocytes, Metabolism Qualified

Catalog number:  HMCPMS

Related applications:

Pharma & Biopharma

| Primary Cell Culture

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Description

These cryopreserved human hepatocytes are suitable for use in plated metabolism applications.

Cells per vial: 4-8 million

Prototypical cytochrome P450 substrates midazolam, tolbutamide, and dextromethorphan were used to assess the enzymatic function of CYP3A4⁄5, CYP2C9 and CYP2D6 respectively. The disappearance of parent was monitored by LC-MS⁄MS analysis and intrinsic clearance (CLint) values determined by linear regression.

Get the Results You Need with GIBCO Hepatocytes
High quality hepatocytes, with high viabilities, proper cell morphology, and metabolic activity increase the ability to draw in vitro⁄in vivo correlations and make sound decisions regarding a compound's fate. Experienced technicians and proprietary isolation techniques combined with stringent release specifications ensure you receive the highest quality cryopreserved hepatocytes.

Each lot is tested for:
• Viability ≥ 70%
• Attachment efficiency
• Monolayer formation and integrity
• Confluency >70% on Day 2
• Tested for plating in 96 well format
• Phase I enzyme activities
• Relevant CYP450 polymorphisms

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Note:
These products require shipment in LN2 vapor dewars therefore additional fees may apply.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Form: Cryopreserved
No. of Cells: 4-8 x 106
Product Line: Gibco®
Donor Source: Single Donor
Species: Human
Quantity: 1 vial
Cell Type: Hepatocytes

Contents & storage

Store in a cryostorage dewar or -135°C freezer.