Immunohistochemical localization of inhibin/activin alpha, betaA and betaB subunits and follistatin in bovine oocytes during in vitro maturation and fertilization.
AuthorsSilva CC, Groome NP, Knight PG
JournalReproduction
PubMed ID12622694
'The aim of this study was to evaluate the distribution of inhibin/activin alpha, beta(A) and beta(B) subunits and follistatin in immature oocytes and in matured oocytes before and after IVF. Denuded oocytes were submitted to a whole-mount immunofluorescence procedure. Specimens were imaged and fluorescent intensities quantified by scanning laser confocal ... More
Quantitative fluorescence imaging analysis for cancer biomarker discovery: application to beta-catenin in archived prostate specimens.
AuthorsHuang D, Casale GP, Tian J, Wehbi NK, Abrahams NA, Kaleem Z, Smith LM, Johansson SL, Elkahwaji JE, Hemstreet GP,
JournalCancer Epidemiol Biomarkers Prev
PubMed ID17623804
The surprising disparity between the number of protein-encoding genes ( approximately 30,000) in the human genome and the number of proteins ( approximately 300,000) in the human proteome has inspired the development of translational proteomics aimed at protein expression profiling of disease states. Translational proteomics, which offers the promise of ... More
Quantization of widefield fluorescence images using structured illumination and image analysis software.
AuthorsBarlow AL, Guerin CJ
JournalMicrosc Res Tech
PubMed ID17131356
It is difficult to obtain precise quantitative measurements from fluorescent images captured from widefield microscopes. We wished to ascertain if reliable quantitative measurements of both biological and nonbiological specimens were possible using a widefield microscope equipped with a structured illumination system and image analysis software. In a nonbiological specimen, images ... More
Macaque trophoblast migration is regulated by RANTES.
In human and non-human primates, migratory trophoblasts penetrate the uterine epithelium, invade the endometrium, enter the uterine vasculature, and migrate within the arteries. The mechanisms that regulate this directional migration are unknown. We have used early gestation macaque trophoblasts to test the hypothesis that trophoblast migration is regulated by the ... More
Highly Sensitive Shack-Hartmann Wavefront Sensor: Application to Non-Transparent Tissue Mimic Imaging with Adaptive Light-Sheet Fluorescence Microscopy.
AuthorsMorgado Brajones J, Clouvel G, Dovillaire G, Levecq X, Lorenzo C
JournalMethods Protoc
PubMed ID31336779
'High-quality in-depth imaging of three-dimensional samples remains a major challenge in modern microscopy. Selective plane illumination microscopy (SPIM) is a widely used technique that enables imaging of living tissues with subcellular resolution. However, scattering, absorption, and optical aberrations limit the depth at which useful imaging can be done. Adaptive optics ... More
Confocal imaging capacity on a widefield microscope using a spatial light modulator.
Authors
JournalPLoS One
PubMed ID33591984
Ouabain increases neuronal branching in hippocampus and improves spatial memory.
AuthorsOrellana AM, Leite JA, Kinoshita PF, Vasconcelos AR, Andreotti DZ, de Sá Lima L, Xavier GF, Kawamoto EM, Scavone C
JournalNeuropharmacology
PubMed ID30099050
Previous research shows Ouabain (OUA) to bind Na, K-ATPase, thereby triggering a number of signaling pathways, including the transcription factors NF?B and CREB. These transcription factors play a key role in the regulation of BDNF and WNT-ß-catenin signaling cascades, which are involved in neuroprotection and memory regulation. This study investigated ... More