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Invitrogen™

Image-iT™ Green Hypoxia Reagent

Catalog number:  I14833

 Related applications:

Cell Viability, Proliferation & Function

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Description

Image-iT Green Hypoxia Reagent is a novel, fixable fluorogenic compound for measuring hypoxia in live cells. It is non-fluorescent when live cells are in an environment with normal oxygen concentrations and becomes fluorescent when oxygen levels are decreased. Image-iT Green Hypoxia Reagent sustains its fluorescence when cells/tissue return to normal oxygen levels, allowing the cells/tissue to be fixed with minimal loss of fluorescent signal.

Features of Image-iT Green Hypoxia Reagent include:
• Indicates hypoxia in live cells by fluorescing in low oxygen environments
• Fixable—sustains fluorescent signal when cells/tissue return to normal oxygen levels
• Easy to use—just add to cell culture media and image

Image-iT Green Hypoxia Reagent is a fluorogenic compound that is live cell permeable and becomes fluorescent in environments with low oxygen concentrations. These properties makes it a highly useful tool for detecting cells and tissue under hypoxic conditions. Image-iT Green Hypoxia Reagent is a very sensitive oxygen detector. Unlike pimonidazole adducts that respond only to very low oxygen levels, Image-iT Green Hypoxia Reagent begins to fluoresce when atmospheric oxygen levels are less than 5%. It responds quickly to such environments, and the fluorescence is sustained after the oxygen levels return to normal. These properties make Image-iT Green Hypoxia Reagent an ideal tool for detecting hypoxic conditions around tumors, 3D cultures, spheroids, neurons, etc.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Reagent Type: Hypoxia Reagent
Product Line: Image-iT™
Product Size: 5 vials
Shipping Condition: Approved for shipment on Wet or Dry Ice

Contents & storage

Store at -5 to -30°C. Protect from light.

Stable for at least 6 months after receiving.

Documents

Manuals & protocols