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Invitrogen™

ImaGene Red™ C12RG lacZ Gene Expression Kit

Catalog number:  I2906

Related applications:

Cellular Imaging

| Protein Assays and Analysis

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Description

The ImaGene Red™ lacZ Gene Expression Kit contains a fluorescein-based galactosidase substrate that has been covalently modified to include a 12-carbon lipophilic moiety, C12RG. Once inside the cell, the substrates are cleaved by β-galactosidase, producing a fluorescent product that is well retained by the cells, probably by incorporation of the lipophilic tail within the cellular membrane. In addition to the C12RG, the kits also include the broad-spectrum β-galactosidase inhibitor, PETG, and chloroquine diphosphate for inhibiting acidic hydrolysis of the substrate.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Assay: β-Gal (lacZ) Assay
Excitation/Emission: 571⁄585
Product Line: ImaGene Green™
Shipping Condition: Room Temperature
Substrate: C12RG
Substrate Properties: Chemical Substrate
Substrate Type: Beta-Gal Substrate
Target Enzyme: Beta-Galactosidase
Detection Method: Fluorescent

Contents & storage

Store in freezer (-5 to -30°C) and protect from light.