The Image-iT™ LIVE Lysosomal and Nuclear Labeling Kit provides two stains - red-fluorescent LysoTracker® Red DND-99 dye and blue-fluorescent Hoechst 33342 dye - for highly selective staining of lysosomes and the nucleus, respectively, in live green-fluorescent protein (GFP)-transfected cells. When used according to the sample protocol, cell-permeant LysoTracker Red DND-99 provides highly selective lysosomal staining with minimal background. Hoechst 33342 dye, a cell-permeant nucleic acid stain that is selective for DNA and spectrally similar to DAPI, is UV excitable and emits blue fluorescence when bound to DNA. This dye should not interfere with GFP fluorescence and is retained after fixation and permeabilization.
For Research Use Only. Not for use in diagnostic procedures.