Use ProLong® Live Antifade Reagent for brightest fluorescent images with live cells

Image-iT® LIVE Red Caspase-3 and -7 Detection Kit, for microscopy

Catalog number: I35102

Molecular Probes™  Related applications: Cell Viability, Proliferation & Function | Cellular Imaging | Pharma & Biopharma | Target-Based ADME⁄Tox Assays

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Description

The Image-iT™ LIVE Red Caspase-3 and -7 Detection Kit employs a novel approach to detect active caspases that is based on a fluorescent inhibitor of caspases (FLICA™) methodology, essentially an affinity label. The reagent associates a fluoromethyl ketone (FMK) moiety, which can react covalently with a cysteine, with a caspase-specific amine acid sequence. For caspase-3 and -7, this recognition sequence is aspartic acid-glutamic acid-valine-aspartic acid (DEVD). A sulforhodamine group (SR) is attached as a reporter. The FLICA reagent is thought to interact with the enzymatic reactive center of an activated caspase via the recognition sequence, and then to attach covalently through the FMK moiety. The FLICA inhibitor is cell permeant and noncytotoxic. Unbound FLICA molecules diffuse out of the cell and are washed away; the remaining red-fluorescent signal is a direct measure of the amount of active caspase that was present at the time the inhibitor was added.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Detection Method: Fluorescent
For Use With (Equipment): Fluorescence Microscope
Format: Slide(s)
Label or Dye: Hoechst 33342, SR-DEVD-FMK, SYTOX® Green
Product Size: 1 kit
Shipping Condition: Room Temperature

Contents & storage

Store in refrigerator (2–8°C) and protect from light.

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