The T-cell antigen receptor regulates sustained increases in cytoplasmic free Ca2+ through extracellular Ca2+ influx and ongoing intracellular Ca2+ mobilization.
AuthorsImboden JB, Weiss A
JournalBiochem J
PubMed ID3426556
Signal transduction by the T-cell antigen receptor involves the turnover of polyphosphoinositides and an increase in the concentration of cytoplasmic free Ca2+ ([Ca2+]i). This increase in [Ca2+]i is due initially to the release of Ca2+ from intracellular stores, but is sustained by the influx of extracellular Ca2+. To examine the ... More
Trp1, a candidate protein for the store-operated Ca(2+) influx mechanism in salivary gland cells.
AuthorsLiu X, Wang W, Singh BB, Lockwich T, Jadlowiec J, O'Connell B, Wellner R, Zhu MX, Ambudkar IS
JournalJ Biol Chem
PubMed ID10652333
'The trp gene family has been proposed to encode the store-operated Ca(2+) influx (SOC) channel(s). This study examines the role of Trp1 in the SOC mechanism of salivary gland cells. htrp1, htrp3, and Trp1 were detected in the human submandibular gland cell line (HSG). HSG cells stably transfected with htrp1alpha ... More
Mechanisms of subcellular cytosolic Ca2+ signaling evoked by stimulation of the vasopressin V1a receptor.
'Receptor activation may result in distinct subcellular patterns of Ca2+ release. To define the subcellular distribution of Ca2+i signals induced by stimulation of the vasopressin V1a receptor, we expressed the cloned receptor in Xenopus oocytes. Oocytes were then loaded with fluo-3 and observed using confocal microscopy. Vasopressin induced a single ... More
Caffeine inhibits inositol trisphosphate-mediated liberation of intracellular calcium in Xenopus oocytes.
AuthorsParker I, Ivorra I
JournalJ Physiol
PubMed ID1844813
'1. Voltage-clamp recording of Ca(2+)-activated chloride currents in Xenopus oocytes was used to study the effects of caffeine on the liberation of intracellular Ca2+ induced by photo-release of inositol 1,4,5-trisphosphate (InsP3) from caged InsP3. Bath application of caffeine, at concentrations between 0.1 and 10 mM, reduced or abolished the current ... More
Kinetics of calcium release by immunoaffinity-purified inositol 1,4,5-trisphosphate receptor in reconstituted lipid vesicles.
AuthorsHirota J, Michikawa T, Miyawaki A, Furuichi T, Okura I, Mikoshiba K
JournalJ Biol Chem
PubMed ID7642567
'The kinetics of inositol 1,4,5-trisphosphate (IP3)-induced Ca2+ release of the immunoaffinity-purified IP3 receptor (IP3R), reconstituted into lipid vesicles, was investigated using the fluorescent Ca2+ indicator fluo-3. IP3R was purified from mouse cerebellar microsomal fraction by using an immunoaffinity column conjugated with an anti-IP3R type 1 (IP3R1) antibody. The immunoblotting analysis ... More
Regulation of the rate and extent of phospholipase C beta 2 effector activation by the beta gamma subunits of heterotrimeric G proteins.
AuthorsRunnels LW, Scarlata SF
JournalBiochemistry
PubMed ID9799521
'The activity of mammalian phosphoinositide-specific phospholipase C beta 2 (PLC-beta 2) is regulated by the alpha q family of G proteins and by beta gamma subunits. We measured the affinity between the laterally associating PLC-beta 2 and G beta gamma on membrane surfaces by fluorescence resonance energy transfer. Using a ... More
Signal transduction during exocytosis in Limulus polyphemus granulocytes.
AuthorsSolon E, Gupta AP, Gaugler R
JournalDev Comp Immunol
PubMed ID9016385
'Bacterial lipopolysaccharide (LPS)-induced exocytosis is one of the primary immune responses of the Limulus granulocyte (GR). Exocytosis can be mediated by guanine nucleotide-binding protein (G-protein)-linked surface receptors that activate phospholipase C (PLC) to produce inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG). IP3 mobilizes intracellular Ca2+ ([Ca2+]i), which can lead to exocytosis. ... More
Different localization of inositol 1,4,5-trisphosphate and ryanodine binding sites in rat liver.
AuthorsFeng L, Pereira B, Kraus-Friedmann N
JournalCell Calcium
PubMed ID1321686
'The distribution of inositol 1,4,5-trisphosphate and ryanodine binding sites between plasma membrane, microsomal, and mitochondrial fractions of rat liver were compared. IP3 bound mostly to the plasma membrane fraction (Kd = 6 nM; Bmax = 802 fmol/mg protein). Some IP3 binding sites were also present in the microsomal and mitochondrial ... More
Functional screening of intracellular proteins in single cells and in patterned cell arrays using electroporation.
AuthorsNolkrantz K, Farre C, Hurtig KJ, Rylander P, Orwar O
JournalAnal Chem
PubMed ID12199607
'A tool for detection and characterization of intracellular enzyme-substrate and receptor-ligand interactions inside the cytoplasm of single targeted cells or small confined groups of cells is presented. Fluorogenic enzyme substrates and receptor ligands were rapidly delivered by electroosmosis and internalized by electroporation in cells using an electrolyte-filled capillary (EFC) biased ... More
Picomolar platelet-activating factor mobilizes Ca to change platelet shape without activating phospholipase C or protein kinase C; simultaneous fluorometric measurement of intracellular free Ca concentration and aggregation.
AuthorsJames-Kracke MR, Sexe RB, Shukla SD
JournalJ Pharmacol Exp Ther
PubMed ID7965802
'The purpose of this study was to investigate signal transduction mechanisms activated by low and high concentrations of platelet-activating factor (PAF) in rabbit platelets and to contrast the responses to those induced by thrombin. We measured changes in intracellular free calcium ([Ca++]i) with fura2, while monitoring light scatter simultaneously as ... More
Stimulation of Ca2+ efflux from fura-2-loaded platelets activated by thrombin or phorbol myristate acetate.
AuthorsPollock WK, Sage SO, Rink TJ
JournalFEBS Lett
PubMed ID3792558
'We investigated the restoration of [Ca2+]i in fura-2-loaded human platelets following discharge of internal Ca2+ stores in the absence of external Ca2+. After stimulation by thrombin [Ca2+]i returned from a peak level of 0.6 microM to resting levels within 4 min. When ionomycin discharged the internal stores the recovery was ... More
Guanine nucleotide- and inositol 1,4,5-trisphosphate-induced calcium release in rabbit main pulmonary artery.
AuthorsKobayashi S, Somlyo AP, Somlyo AV
JournalJ Physiol
PubMed ID3150985
'1. The effects of activation of guanine nucleotide-binding protein (G protein) by guanine nucleotides or sodium fluoride on the release of intracellular Ca2+ and on tension development were determined in chemically skinned strips of rabbit main pulmonary arteries (MPA). Ca2+ movements were monitored with Fura-2, as the change in free ... More
Inositol 1,4,5-trisphosphate activates receptor-mediated calcium entry by two different pathways in hepatocytes.
AuthorsStriggow F, Bohnensack R
JournalEur J Biochem
PubMed ID8200348
'The quenching of fura-2 fluorescence by the influx of extracellular Mn2+ was measured to indicate the flux rates through receptor-operated calcium channels in the plasma membrane of rat hepatocytes. Neomycin, an inhibitor of phospholipase C, inhibited the vasopressin-induced influx of Mn2+. Thus, the agonist-induced entry of extracellular calcium into hepatocytes ... More
Interactions between inositol phosphates and cytosolic free calcium following bradykinin stimulation in cultured human skin fibroblasts.
AuthorsHuang HM, Toral-Barza L, Gibson GE
JournalBiochim Biophys Acta
PubMed ID2001422
'The inositol triphosphate (IP3) that results from hydrolysis of phosphatidylinositol 4,5-bisphosphate (PIP2) is generally accepted to be responsible for the mobilization of intracellular calcium. However, some studies suggest that low concentrations of agonists elevate cytosolic free calcium concentration ([Ca2+]i) without IP3 formation. Thus, in the present studies, a comparison of ... More
Cytosolic calmodulin is increased in SK-N-SH human neuroblastoma cells due to release of calcium from intracellular stores.
AuthorsMcGinnis KM, Shariat-Madar Z, Gnegy ME
JournalJ Neurochem
PubMed ID9422356
'Muscarinic receptor stimulation elicits a redistribution of calmodulin (CaM) from the membrane fraction to cytosol in the human neuroblastoma cell line SK-N-SH. Increasing the intracellular Ca2+ concentration with ionomycin also elevates cytosolic CaM. The aim of this study was to investigate the roles of extracellular and intracellular Ca2+ pools in ... More
Effect of intracellular injection of inositol trisphosphate on cytosolic calcium and membrane currents in Aplysia neurons.
AuthorsLevy S
JournalJ Neurosci
PubMed ID1607931
'Pacemaker cells of Aplysia californica display a regular bursting that results from a complex interplay of Ca(2+)-mediated conductances and a continuous influx and extrusion of Ca2+. The effect of the second messenger 1,4,5-inositol trisphosphate (InsP3) on intracellular free Ca2+ concentration (Cai) regulation and electrical properties was investigated in identified neurons ... More
Agonist-induced endocytosis and signal generation in adrenal glomerulosa cells. A potential mechanism for receptor-operated calcium entry.
'The relationships between receptor-mediated endocytosis and the generation of intracellular signals were analyzed in angiotensin II (AII)-stimulated adrenal glomerulosa cells. In cells equilibrated with 125I-AII analogs at 4 degrees C, specifically bound agonist but not antagonist AII derivatives were rapidly internalized at 37 degrees C. AII-induced internalization was not influenced ... More
Patterns of intracellular Ca2+ concentrations in fertilized bovine eggs.
AuthorsFissore RA, Dobrinsky JR, Balise JJ, Duby RT, Robl JM
JournalBiol Reprod
PubMed ID1493184
'Sperm-induced calcium (Ca2+) changes were examined in zona pellucida-intact, mature bovine eggs injected with the fluorescent Ca2+ indicator fura-2 dextran (fura-2 D). Fifty four percent (37/68) of the dye-injected, inseminated bovine eggs were fertilized and 43% (16/37) of the fertilized eggs exhibited Ca2+ elevations during the time of measurement. All ... More
Subcellular distribution of Ca2+ release channels underlying Ca2+ waves and oscillations in exocrine pancreas.
AuthorsKasai H, Li YX, Miyashita Y
JournalCell
PubMed ID8395348
'Agonists trigger Ca2+ waves and oscillations in exocrine gland cells. Our confocal Ca2+ imaging revealed three distinct phases during the Ca2+ waves in the rat pancreatic acinar cell. Rises in Ca2+ concentration were initiated at a small trigger zone, or T zone, in the granular area; then, Ca2+ waves rapidly ... More
Spatial heterogeneity of caffeine- and inositol 1,4,5-trisphosphate-induced Ca2+ transients in isolated snail neurons.
AuthorsKostyuk PG, Kirischuk SI
JournalNeuroscience
PubMed ID8506027
'Inositol 1,4,5-trisphosphate- and caffeine-induced Ca2+ release was examined in neurons isolated from the mollusc Helix pomatia using Ca2+ indicator fura-2 and fluorescent digital-imaging microscopy technique. Extracellular application of caffeine caused a fast and pronounced augmentation of [Ca2+]i whose amplitude and kinetics differ in the centre of the cell and near ... More
Decreased efficacy of inositol 1,4,5-trisphosphate to elicit calcium mobilization from cerebrocortical microsomes of aged rats.
AuthorsBurnett DM, Daniell LC, Zahniser NR
JournalMol Pharmacol
PubMed ID2157962
'The effects of aging on the ability of brain microsomes to sequester calcium in response to ATP stimulation and to release calcium in response to inositol 1,4,5-trisphosphate (IP3) stimulation were examined. Calcium uptake and release were compared in microsomal preparations from the cerebral cortex, hippocampus, thalamus, and cerebellum of 3-, ... More
Fluoride interactions with stimulus-secretion coupling of normal and pathological parathyroid cells.
'Effects of the GTP binding protein (G-protein) activator NaF on parathyroid hormone (PTH) release, cytoplasmic Ca2+ concentration ([Ca2+]i) and cAMP content of bovine as well as normal and pathological human parathyroid cells were studied using precautions to avoid CaF2 precipitation. In 0.5 mM external Ca2+, NaF inhibited PTH release and ... More
Sperm, inositol trisphosphate, and thimerosal-induced intracellular Ca2+ elevations in rabbit eggs.
AuthorsFissore RA, Robl JM
JournalDev Biol
PubMed ID8365556
'Fertilization-induced calcium (Ca2+) changes were examined in in vivo fertilized rabbit eggs, using the fluorescent Ca2+ inducer fura-2 dextran. Twenty-four of 48 fertilized eggs exhibited repetitive Ca2+ rises with intervals of 13 +/- 1 min (mean +/- SEM) during the recording period of 45 min. None of the unfertilized eggs ... More
Characterization of inositol 1,4,5-trisphosphate-stimulated calcium release from rat cerebellar microsomal fractions. Comparison with [3H]inositol 1,4,5-trisphosphate binding.
AuthorsStauderman KA, Harris GD, Lovenberg W
JournalBiochem J
PubMed ID3264497
'The abilities of D-myo-inositol phosphates (InsPs) to promote Ca2+ release and to compete for D-myo-[3H]-inositol 1,4,5-trisphosphate [( 3H]Ins(1,4,5)P3) binding were examined with microsomal preparations from rat cerebellum. Of the seven InsPs examined, only Ins(1,4,5)P3, Ins(2,4,5)P3 and Ins(4,5)P2 stimulated the release of Ca2+. Ca2+ release was maximal in 4-6 s and ... More
Inositol 1,4,5-trisphosphate-induced calcium mobilization is localized in Xenopus oocytes.
AuthorsBerridge MJ
JournalProc R Soc Lond B Biol Sci
PubMed ID2575752
'Injection of inositol 1,4,5-trisphosphate (Ins(1,4,5)P3) into the animal pole of Xenopus oocytes induced membrane depolarization due to the internal mobilization of calcium, which activates a chloride conductance. Repetitive injections of Ins(1,4,5)P3 results in desensitization probably as a result of depletion of the internal store of calcium. Desensitization was restricted to ... More
Phospholipase C-delta1 is activated by capacitative calcium entry that follows phospholipase C-beta activation upon bradykinin stimulation.
AuthorsKim YH, Park TJ, Lee YH, Baek KJ, Suh PG, Ryu SH, Kim KT
JournalJ Biol Chem
PubMed ID10473563
'To characterize the regulatory mechanism of phospholipase C-delta1 (PLC-delta1) in the bradykinin (BK) receptor-mediated signaling pathway, we used a clone of PC12 cells, which stably overexpress PLC-delta1 (PC12-D1). Stimulation with BK induced a significantly higher Ca(2+) elevation and inositol 1,4,5-trisphosphate (IP(3)) production with a much lower half-maximal effective concentration (EC(50)) ... More
A quantitative investigation into the dependence of Ca2+ mobilisation on changes in inositol 1,4,5-trisphosphate levels in the stimulated neutrophil.
AuthorsThompson NT, Bonser RW, Tateson JE, Spacey GD, Randall RW, Hodson HF, Garland LG
JournalBr J Pharmacol
PubMed ID1884113
'1. The coupling of N-formyl-methionyl-leucyl-phenylalanine (fMet-Leu-Phe) receptor stimulation to Ca2+ mobilisation has been investigated in the human neutrophil by measuring the concentration-effect curves for inositol 1,4,5-trisphosphate (IP3) formation and Ca2+ mobilisation. 2. fMet-Leu-Phe-dependent mobilisation of intracellular Ca2+ has been monitored in fluo-3-loaded human neutrophils by measuring increases in the cytoplasmic ... More
Metabolism of inositol 1,3,4-trisphosphate to a new tetrakisphosphate isomer in angiotensin-stimulated adrenal glomerulosa cells.
AuthorsBalla T, Guillemette G, Baukal AJ, Catt KJ
JournalJ Biol Chem
PubMed ID3497156
'Angiotensin stimulates rapid and prominent increases in inositol polyphosphates and their metabolites in bovine glomerulosa cells labeled with [3H]inositol. In addition to the early formation of inositol 1,4,5-trisphosphate (Ins-1,4,5-P3) and inositol 1,3,4-trisphosphate (Ins-1,3,4-P3), as well as their intermediate product, inositol 1,3,4,5-tetrakisphosphate (Ins-1,3,4,5-P4), delayed increases in two new InsP4 isomers were ... More
Technique for in situ measurement of calcium in intracellular inositol 1,4,5-trisphosphate-sensitive stores using the fluorescent indicator mag-fura-2.
AuthorsHofer AM, Machen TE
JournalProc Natl Acad Sci U S A
PubMed ID8464866
'Stimulation of cells with calcium-mobilizing agonists frequently results in inositol 1,4,5-trisphosphate (InsP3)-mediated discharge of Ca from an internal store. We report here a technique for directly monitoring Ca within this and other stores in gastric epithelial cells. This technique takes advantage of the propensity of the acetoxymethyl ester derivative of ... More
Conversion of PtdIns(4,5)P(2) into PtdIns(5)P by the S.flexneri effector IpgD reorganizes host cell morphology.
'Phosphoinositides play a central role in the control of several cellular events including actin cytoskeleton organization. Here we show that, upon infection of epithelial cells with the Gram-negative pathogen Shigella flexneri, the virulence factor IpgD is translocated directly into eukaryotic cells and acts as a potent inositol 4-phosphatase that specifically ... More
Local positive feedback by calcium in the propagation of intracellular calcium waves.
AuthorsWang SS, Thompson SH
JournalBiophys J
PubMed ID8580312
'In many types of eukaryotic cells, the activation of surface receptors leads to the production of inositol 1,4,5-trisphosphate and calcium release from intracellular stores. Calcium release can occur in complex spatial patterns, including waves of release that traverse the cytoplasm. Fluorescence video microscopy was used to view calcium waves in ... More
Mechanical stimulation and intercellular communication increases intracellular Ca2+ in epithelial cells.
AuthorsSanderson MJ, Charles AC, Dirksen ER
JournalCell Regul
PubMed ID2078569
'Intercellular communication of epithelial cells was examined by measuring changes in intracellular calcium concentration ([Ca2+]i). Mechanical stimulation of respiratory tract ciliated cells in culture induced a wave of increasing Ca2+ that spread, cell by cell, from the stimulated cell to neighboring cells. The communication of these Ca2+ waves between cells ... More
Role of protein kinase C in the regulation of cytosolic Ca2+ in A431 cells: separation of growth factor and bradykinin pathways.
AuthorsWheeler LA, Goodrum DD, Sachs G
JournalJ Membr Biol
PubMed ID2283681
'Calcium signaling systems in nonexcitable cells involve activation of Ca2+ entry across the plasma membrane and release from intracellular stores as well as activation of Ca2+ pumps and inhibition of passive Ca2+ pathways to ensure exact regulation of free cytosolic Ca2+ concentration [( Ca2+]i). A431 cells loaded with fura-2 cells ... More
Inositol 1,4,5-trisphosphate: a possible chemical link in excitation-contraction coupling in muscle.
AuthorsVergara J, Tsien RY, Delay M
JournalProc Natl Acad Sci U S A
PubMed ID2994073
'The role of inositol 1,4,5-trisphosphate (InsP3) in excitation-contraction coupling in skeletal muscle was investigated by several methods. The following results were obtained. InsP3 is released by electrical stimulation of muscles. Exogenous InsP3 releases calcium from skinned muscle fibers at relatively high doses under normal conditions but does so at very ... More
[Effect of heparin on the increase in intracellular calcium caused by inositol 1,4,5-triphosphate in airway epithelial cells]
AuthorsKondo M, Tamaoki J, Isono K, Kanoh S, Konno K
JournalNihon Kyobu Shikkan Gakkai Zasshi
PubMed ID9103854
'We studied the effect of heparin on the inositol 1,4,5-triphosphate (IP3)-dependent increase in intracellular calcium (Ca2+) caused by ATP. The Ca2+ concentration ([Ca2+]i) in cultured epithelial cells from cow tracheas was measured by the fura-2 method. ATP (10(-4) M) stimulated IP3 production and caused a biphasic increase in [Ca2+]i a ... More
Cyclic ADP-ribose and inositol 1,4,5-triphosphate mobilizes Ca2+ from distinct intracellular pools in permeabilized lacrimal acinar cells.
AuthorsGromada J, Jørgensen TD, Dissing S
JournalFEBS Lett
PubMed ID7883052
'In permeabilized lacrimal acinar cells, cyclic ADP-ribose (cADP-ribose) and inositol 1,4,5-trisphosphate (Ins(1,4,5)P3) release Ca2+ in a dose dependent manner from distinct thapsigargin-sensitive Ca2+ pools. Ryanodine specifically blocks the Ca2+ response to cADP-ribose, whereas heparin strongly reduces the response to Ins(1,4,5)P3 application. GTP causes a rapid Ca2+ release by a ryanodine- ... More
Gonadotropin-releasing hormone-induced rise in cytosolic free Ca2+ levels: mobilization of cellular and extracellular Ca2+ pools and relationship to gonadotropin secretion.
AuthorsNaor Z, Capponi AM, Rossier MF, Ayalon D, Limor R
JournalMol Endocrinol
PubMed ID2458525
'Addition of GnRH to pituitary gonadotrophs preloaded with Quin 2 resulted in a rapid (approximately 8 s) mobilization of an ionomycin-sensitive intracellular Ca2+ pool. A second component of Ca2+ entry via voltage dependent channels contributed about 45% of the peak cytosolic free Ca2+ concentration ([Ca2+]i). Thereafter, influx of Ca2+ via ... More
Inositol 1,4,5-trisphosphate induces aggregation and release of 5-hydroxytryptamine from saponin-permeabilized human platelets.
AuthorsWatson SP, Ruggiero M, Abrahams SL, Lapetina EG
JournalJ Biol Chem
PubMed ID3082884
'Inositol 1,4,5-trisphosphate induces aggregation and the release of [3H]5-hydroxytryptamine from human platelets rendered permeable with saponin. This action of inositol 1,4,5-trisphosphate is associated with a significant formation of thromboxane B2, activation of phospholipase C, and phosphorylation of 20,000- and 40,000-dalton proteins, which are the substrates for myosin light chain kinase ... More
Ryanodine and inositol trisphosphate receptors are differentially distributed and expressed in rat parotid gland.
AuthorsZhang X, Wen J, Bidasee KR, Besch HR, Wojcikiewicz RJ, Lee B, Rubin RP
JournalBiochem J
PubMed ID10333498
'The present study examines the cellular distribution of the ryanodine receptor/channel (RyR) and inositol 1,4,5-trisphosphate receptor (InsP3R) subtypes in parotid acini. Using fluorescently labelled 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene-3-propionic acid glycyl-ryanodine (BODIPYtrade mark-ryanodine) and confocal microscopy, RyRs were localized primarily to the perinuclear region (basal pole) of the acinar cell. Ryanodine, Ruthenium Red, cAMP ... More
InsP3-dependent Ca2+ mobilization in circular but not longitudinal muscle cells of intestine.
AuthorsMurthy KS, Grider JR, Makhlouf GM
JournalAm J Physiol
PubMed ID1662915
'The presence and functional coupling of inositol 1,4,5-trisphosphate (InsP3) receptors was examined in permeabilized muscle cells and microsomes isolated separately from the circular and longitudinal muscle layers of guinea pig intestine. Specific binding of [3H]InsP3 occurred only in circular muscle cells and microsomes; binding to longitudinal muscle cells or microsomes ... More
Evidence of Ca2+ mobilizing action of arachidonic acid in human platelets.
AuthorsTohmatsu T, Nakashima S, Nozawa Y
JournalBiochim Biophys Acta
PubMed ID2499358
'The addition of arachidonic acid induced a rapid release of 45Ca2+ from human platelet membrane vesicles which accumulated 45Ca2+ in the presence of ATP. Docosahexaenoic acid, eicosapentaenoic acid, linolenic acid and linoleic acid were less active than arachidonic acid. In contrast, oleic acid, myristic acid and palmitic acid were without ... More
Spontaneous calcium release from inositol trisphosphate-sensitive calcium stores.
AuthorsMissiaen L, Taylor CW, Berridge MJ
JournalNature
PubMed ID1857419
'Inositol 1,4,5-trisphosphate (InsP3) functions as a second messenger to mobilize Ca2+ from intracellular reservoirs. The release mechanism displays all-or-none characteristics, that may account for other observations that the InsP3-induced mobilization of Ca2+ is quantal. Quantal release may depend on the sensitivity of the InsP3 receptor being regulated by the Ca2+ ... More
LTD4 increases cytosolic free calcium and inositol phosphates in human neutrophils: inhibition by the novel LTD4 receptor antagonist, SR2640, and possible relation to modulation of chemotaxis.
AuthorsBouchelouche PN, Ahnfelt-Rønne I, Thomsen MK
JournalAgents Actions
PubMed ID2160189
'LTD4 increased the level of free intracellular calcium ([Ca++]i) and stimulated the production of inositol phosphates (IP) in human polymorphonuclear neutrophils (PMN). Calcium was predominantly mobilized from intracellular pools. After a single stimulus, the cells were refractory to a second challenge with the same concentration of LTD4, but the calcium ... More
cis,cis-cyclohexane 1,3,5-triol polyphosphates release calcium from Neurospora crassa via an unspecific Ins 1,4,5-P3 receptor.
AuthorsSchultz C, Gebauer G, Metschies T, Rensing L, Jastorff B
JournalBiochem Biophys Res Commun
PubMed ID2154977
'We investigated the effects of new inositol 1,4,5-trisphosphate analogues on the release of Ca2+ from isolated vacuoles of Neurospora crassa. Tri-O-butyryl-inositol 1,4,5-trisphosphate and a set of cis,cis-cyclohexane 1,3,5-triol bis-(CHT-P2) and trisphosphates (CHT-P3) gave an increase in free Ca2+ as measured directly with fura-2, a Ca2(+)-chelator. However, inositol 1,4-bisphosphate, 6-O-palmitoyl-inositol 4,5-bisphosphate ... More
Characterization of Ca2+ transients induced by intracellular photorelease of InsP3 in mouse ovarian oocytes.
AuthorsPeres A, Bertollini L, Racca C
JournalCell Calcium
PubMed ID1934036
'Ca2+ transients (measured with Fluo-3) were induced in single mouse ovarian oocytes by photolytic liberation of InsP3. The time course of cytosolic Ca2+ changes induced in this way is composed of distinct phases: upstroke, fast decline, slow declining plateau and fast decline to rest level. All the phases reflect mainly ... More
Does the nuclear envelope contain two types of ligand-gated Ca2+ release channels?
AuthorsGuihard G, Proteau S, Rousseau E
JournalFEBS Lett
PubMed ID9305738
'The nuclear envelope is composed of two membranes deliminating a perinuclear space which displays functional properties similar to those of a Ca2+-storing compartment. ATP-driven Ca2+ uptake and InsP3-induced Ca2+ release processes have been described in isolated nuclei. Recently, it was reported that cADP-ribose and InsP3 can trigger a nucleoplasmic Ca2+ ... More
Inositol tetrakisphosphate-induced sequestration of Ca2+ replenishes an intracellular pool sensitive to inositol trisphosphate.
AuthorsHill TD, Boynton AL
JournalJ Cell Physiol
PubMed ID2298819
'In a permeable neoplastic rat liver epithelial (261B) cell system, inositol 1,3,4,5-tetrakisphosphate--Ins(1,3,4,5)P4--induces sequestration of Ca2+ released by inositol 2,4,5-trisphosphate--Ins(2,4,5)P3; a non-metabolized inositol trisphosphate (InsP3) isomer--and Ca2+ added exogenously in the form of CaCl2. Studies were performed to identify the Ca2+ pool filled after Ins(1,3,4,5)P4 treatment. Both Ins(2,4,5)P3 and inositol 1,4,5-trisphosphate--Ins(1,4,5)P3--dose-dependently ... More
Inositol 1,4,5-trisphosphate induced Ca2+ release from chloroquine-sensitive and -insensitive intracellular stores in the intraerythrocytic stage of the malaria parasite P. chabaudi.
AuthorsPassos AP, Garcia CR
JournalBiochem Biophys Res Commun
PubMed ID9535800
'Isolated P. chabaudi parasites were permeabilized with digitonin and the function of intracellular Ca2+ stores was studied using the Ca2+ indicators arsenazo III or Fluo 3-acid in the medium. Addition of the second messenger InsP3 (5 microM) to permeabilized parasites leads to Ca2+ release into the medium, with the mean ... More
Ca2+ release induced by inositol 1,4,5-trisphosphate is a steady-state phenomenon controlled by luminal Ca2+ in permeabilized cells.
AuthorsMissiaen L, De Smedt H, Droogmans G, Casteels R
JournalNature
PubMed ID1608471
'Low concentrations of inositol 1,4,5-trisphosphate (InsP3) evoke a very rapid mobilization of intracellular Ca2+ stores in many cell types, which can be followed by a further, much slower efflux. Two explanations have been suggested for this biphasic release. The first proposes that the Ca2+ stores vary in their sensitivity to ... More
Presence of m3 subtype muscarinic acetylcholine receptors and receptor-mediated increases in the cytoplasmic concentration of Ca2+ in Jurkat, a human leukemic helper T lymphocyte line.
AuthorsKaneda T, Kitamura Y, Nomura Y
JournalMol Pharmacol
PubMed ID8383801
'Recent studies have demonstrated the presence and the regulatory function of several neurotransmitters in the immune system. In the present study, we examined the presence of acetylcholine receptors, using pharmacological and molecular biological assays, and their transmembrane control and functions, using a biochemical assay, in a cloned human leukemic helper ... More
Localized superoxide release by neutrophils can be provoked by a cytosolic calcium 'cloud'.
AuthorsDavies EV, Hallett MB, Campbell AK
JournalImmunology
PubMed ID1649127
'We have used single-cell ratio imaging of Fura-2 loaded neutrophils to visualize release of cytosolic Ca2+ from an intracellular store in order to determine the location of this store and the relationship of release from it to oxidase activation. In the presence of extracellular Ca2+, N-formyl-L-methionyl-L-leucyl-L-phenylalanine (FMLP) produced an increase ... More
Back in the water: the return of the inositol phosphates.
AuthorsIrvine RF, Schell MJ
JournalNat Rev Mol Cell Biol
PubMed ID11331907
'Following the discovery of inositol-1,4,5-trisphosphate as a second messenger, many other inositol phosphates were discovered in quick succession, with some understanding of their synthesis pathways and a few guesses at their possible functions. But then it all seemed to go comparatively quiet, with an explosion of interest in the inositol ... More
CCK-JMV-180, an analog of cholecystokinin, releases intracellular calcium from an inositol trisphosphate-independent pool in rat pancreatic acini.
AuthorsSaluja AK, Dawra RK, Lerch MM, Steer ML
JournalJ Biol Chem
PubMed ID1375937
'In pancreatic acinar cells cholecystokinin and its analogs, caerulein and CCK-JMV-180, stimulate an increase in intracellular free [Ca2+] by releasing Ca2+ from non-mitochondrial intracellular pools. It is generally believed that the caerulein-induced release of Ca2+ is mediated by phospholipase C-catalyzed production of 1,4,5-inositol triphosphate (IP3). In this study we have ... More
The effect of fetal calf serum on intracellular calcium in GH3 cells.
AuthorsWrench IJ, Brown TJ, Brown BL, Dobson PR
JournalBiochem Biophys Res Commun
PubMed ID3142472
'We have investigated the mechanism of action of fetal calf serum (FCS) on GH3 pituitary tumour cells by measuring intracellular free calcium levels. On the addition of FCS (1%) there was a transient increase in intracellular Ca2+ levels which was attenuated in conditions of reduced extracellular calcium concentrations. The Ca2+ ... More
Inositol trisphosphate and calcium signalling.
AuthorsBerridge MJ
JournalNature
PubMed ID8381210
'Inositol trisphosphate is a second messenger that controls many cellular processes by generating internal calcium signals. It operates through receptors whose molecular and physiological properties closely resemble the calcium-mobilizing ryanodine receptors of muscle. This family of intracellular calcium channels displays the regenerative process of calcium-induced calcium release responsible for the ... More
Internal perfusion of guinea-pig hepatocytes with buffered Ca2+ or inositol 1,4,5-trisphosphate mimics noradrenaline activation of K+ and Cl- conductances.
AuthorsCapiod T, Field AC, Ogden DC, Sandford CA
JournalFEBS Lett
PubMed ID2439376
'External application of noradrenaline to voltage-clamped guinea-pig isolated hepatocytes evoked membrane conductance increases to K+ and Cl-. This effect was reproduced by internal perfusion of the cells with 2 microM buffered Ca2+ and with 20 microM inositol 1,4,5-trisphosphate (IP3). The kinetic properties of the K+ conductance and its selective block ... More
Qualitative differences in [Ca2+]i increases and InsP3 generation following stimulation of N1E-115 cells with micromolar and millimolar ATP.
AuthorsIredale PA, Martin KF, Alexander SP, Hill SJ, Kendall DA
JournalBiochem Pharmacol
PubMed ID1417971
'Addition of ATP (100 microM) to populations of the neuronal cell line N1E-115 caused a transient increase in intracellular calcium ([Ca2+]i) which rapidly reached a peak (maximum of 243 +/- 7 nM above basal) and returned to basal after approximately 50 sec. The response was concentration-dependent (EC50 21 +/- 4 ... More
Heat acclimation and heat stress have different effects on cholinergic-induced calcium mobilization.
AuthorsKaspler P, Horowitz M
JournalAm J Physiol Regul Integr Comp Physiol
PubMed ID11353672
'There is evidence that the signal transduction array responsible for the secretion of water in evaporative cooling by the submaxillary gland of the rat is subject to heat acclimatory responses. The objectives of the present study were 1) to examine whether heat acclimation affects intracellular Ca(2+) mobilization and, in turn, ... More
A zinc-sensing receptor triggers the release of intracellular Ca2+ and regulates ion transport.
AuthorsHershfinkel M, Moran A, Grossman N, Sekler I
JournalProc Natl Acad Sci U S A
PubMed ID11573009
'Changes in extracellular zinc concentration participate in modulating fundamental cellular processes such as proliferation, secretion, and ion transport in a mechanism that is not well understood. Here, we show that a micromolar concentration of extracellular zinc triggers a massive release of calcium from thapsigargin-sensitive intracellular pools in the colonocytic cell ... More
Glucose regulation of free Ca(2+) in the endoplasmic reticulum of mouse pancreatic beta cells.
AuthorsTengholm A, Hellman B, Gylfe E
JournalJ Biol Chem
PubMed ID10601240
'Free Ca(2+) was measured in organelles of individual mouse pancreatic beta cells loaded with the low affinity indicator furaptra. After removal of cytoplasmic indicator by controlled digitonin permeabilization the organelle Ca(2+) was located essentially in the endoplasmic reticulum (ER), >90% being sensitive to inhibition of sarco(endo)plasmic reticulum Ca(2+)-ATPases. The Ca(2+) ... More
A novel monovalent cation channel activated by inositol trisphosphate in the plasma membrane of rat megakaryocytes.
AuthorsSomasundaram B, Mahaut-Smith MP
JournalJ Biol Chem
PubMed ID7542650
'The activation of a monovalent cation current was studied in rat megakaryocytes using patch clamp techniques combined with photometric measurements of intracellular concentrations of Ca2+ ([Ca2+]i) and Na+. ADP evoked a release of [Ca2+]i and transiently activated a monovalent cation-selective channel, which, at negative potentials and under physiological conditions, would ... More
A bimodal pattern of InsP(3)-evoked elementary Ca(2+) signals in pancreatic acinar cells.
AuthorsFogarty KE, Kidd JF, Tuft RA, Thorn P
JournalBiophys J
PubMed ID10777728
'InsP(3)-evoked elementary Ca(2+) release events have been postulated to play a role in providing the building blocks of larger Ca(2+) signals. In pancreatic acinar cells, low concentrations of acetylcholine or the injection of low concentrations of InsP(3) elicit a train of spatially localized Ca(2+) spikes. In this study we have ... More
Cytoplasmic Ca2+ oscillations evoked by receptor stimulation, G-protein activation, internal application of inositol trisphosphate or Ca2+: simultaneous microfluorimetry and Ca2+ dependent Cl- current recording in single pancreatic acinar cells.
AuthorsOsipchuk YV, Wakui M, Yule DI, Gallacher DV, Petersen OH
JournalEMBO J
PubMed ID1690123
'The effects of acetylcholine (ACh), cholecystokinin (CCK), internally applied GTP-gamma-S, inositol trisphosphate [Ins (1,4,5) P3] or Ca2+ on the cytoplasmic free Ca2+ concentration [( Ca2+]i) were assessed by simultaneous microfluorimetry (fura-2) and measurement of the Ca2(+)-dependent Cl- current (patch-clamp whole-cell recording) in single internally perfused mouse pancreatic acinar cells. ACh ... More
Influence of nitric oxide on agonist-mediated calcium mobilization in platelets.
AuthorsRao GH, Krishnamurthi S, Raij L, White JG
JournalBiochem Med Metab Biol
PubMed ID2383431
'Recent studies have characterized endothelium-derived relaxing factor as nitric oxide. It appears to exert its effect by elevating intracellular levels of cyclic GMP. In this study we confirm that nitric oxide is a potent inhibitor of agonist-induced irreversible aggregation. At the concentrations tested nitric oxide effectively blocked thrombin-stimulated mobilization of ... More
Bradykinin inhibits a potassium M-like current in rat pheochromocytoma PC12 cells.
AuthorsVillarroel A, Marrion NV, Lopez H, Adams PR
JournalFEBS Lett
PubMed ID2792371
'We studied the action of bradykinin (BK) on ionic currents in fused pheochromocytoma PC12 cells under voltage-clamp in whole-cell mode, and on intracellular calcium using fura-2 BK induced the development of an outward current associated with an increase in intracellular calcium, followed by inhibition of an M-like current. The outward ... More
Inositol trisphosphate and diacylglycerol: two interacting second messengers.
AuthorsBerridge MJ
JournalAnnu Rev Biochem
PubMed ID3304132
Receptor activation and inositol lipid hydrolysis in neural tissues.
AuthorsFisher SK, Agranoff BW
JournalJ Neurochem
PubMed ID3029333
Neurotransmitter receptors and phosphoinositide turnover.
AuthorsChuang DM
JournalAnnu Rev Pharmacol Toxicol
PubMed ID2543275
Inositol phosphates: a family of signal molecules?
AuthorsDownes CP
JournalTrends Neurosci
PubMed ID2469189
Oscillating intracellular Ca2+ signals evoked by activation of receptors linked to inositol lipid hydrolysis: mechanism of generation.
AuthorsPetersen OH, Wakui M
JournalJ Membr Biol
PubMed ID2176239
Inositol polyphosphates and neuronal calcium homeostasis.
AuthorsNahorski SR
JournalTrends Neurosci
PubMed ID2469162
Toward the mechanism of phosphoinositide-specific phospholipases C.
AuthorsBruzik KS, Tsai MD
JournalBioorg Med Chem
PubMed ID7922124
Ca2+ and activation mechanisms in skeletal muscle.
AuthorsAshley CC, Mulligan IP, Lea TJ
JournalQ Rev Biophys
PubMed ID2047521
Inositol trisphosphate induces calcium release from Neurospora crassa vacuoles.
AuthorsCornelius G, Gebauer G, Techel D
JournalBiochem Biophys Res Commun
PubMed ID2527035
Inositol 1,4,5-trisphosphate is known to release calcium ions from intracellular stores thought to be parts of endoplasmic reticulum in animal cells. In Neurospora crassa, however, inositol 1,4,5-trisphosphate acts on vacuoles stimulating a calcium efflux with a Km of 5.28 microM. The calcium release is inhibited effectively by dantrolene. These results ... More
Inositol phosphates and cell signalling.
AuthorsBerridge MJ, Irvine RF
JournalNature
PubMed ID2550825
Inositol 1,4,5-trisphosphate is a second messenger which regulates intracellular calcium both by mobilizing calcium from internal stores and, perhaps indirectly, by stimulating calcium entry. In these actions it may function with its phosphorylated metabolite, inositol 1,3,4,5-tetrakisphosphate. The subtlety of calcium regulation by inositol phosphates is emphasized by recent studies that ... More
A muscarinic receptor agonist mobilizes Ca2+ from caffeine and inositol-1,4,5-trisphosphate-sensitive Ca2+ stores in cat adrenal chromaffin cells.
AuthorsSorimachi M, Yamagami K, Nishimura S
JournalBrain Res
PubMed ID1611489
To gain some understanding of the characteristics of intracellular Ca2+ stores of cat adrenal chromaffin cells, we investigated the effects of ryanodine, a blocker of Ca(2+)-induced Ca2+ release channels in muscle, on both cytosolic Ca2+ concentration and catecholamine secretion induced by caffeine or methacholine. The results suggest that Ca2+ stores ... More
The versatility and universality of calcium signalling.
AuthorsBerridge MJ, Lipp P, Bootman MD
JournalNat Rev Mol Cell Biol
PubMed ID11413485
The universality of calcium as an intracellular messenger depends on its enormous versatility. Cells have a calcium signalling toolkit with many components that can be mixed and matched to create a wide range of spatial and temporal signals. This versatility is exploited to control processes as diverse as fertilization, proliferation, ... More
Role of inositol trisphosphate as a second messenger in signal transduction processes: an essay.
AuthorsOsborne NN, Tobin AB, Ghazi H
JournalNeurochem Res
PubMed ID2838762
This essay attempts to summarize some of the best evidence for the role of inositol trisphosphate as a second messenger in signal transduction processes. The following aspects are addressed in the essay: (a) The synthesis of inositol trisphosphate and other inositol lipids, (b) Receptor-phosphatidylinositol bisphosphate phospholipase C coupling and the ... More
Phagocytosis in Acanthamoeba: II. Soluble and insoluble mannose-rich ligands stimulate phosphoinositide metabolism.
AuthorsAllen PG, Dawidowicz EA
JournalJ Cell Physiol
PubMed ID2177061
The generation of second messengers during phagocytosis of yeast by Acanthamoeba castellanii was examined. The kinetics of binding and internalization of yeast by Acanthamoeba were measured and this was compared with the generation of known second messengers. We observed stimulated degradation of PI-4, 5-P2 to 1,4,5 IP3 with kinetics similar ... More
Calcium dependent inositol trisphosphate-induced calcium release in the guinea-pig taenia caeci.
AuthorsIino M
JournalBiochem Biophys Res Commun
PubMed ID3493000
The properties of the Ca stores in the skinned fiber bundles of the guinea-pig taenia caeci were studied by a novel method to measure Ca released from the stores using a fluorescent Ca indicator, fura-2. It is found that the capacity of the Ca stores amounts some 170 mumoles/liter cell ... More
Spatiotemporal dynamics of inositol 1,4,5-trisphosphate that underlies complex Ca2+ mobilization patterns.
AuthorsHirose K, Kadowaki S, Tanabe M, Takeshima H, Iino M
JournalScience
PubMed ID10348740
Inositol 1,4,5-trisphosphate (IP3) is a second messenger that elicits complex spatiotemporal patterns of calcium ion (Ca2+) mobilization and has essential roles in the regulation of many cellular functions. In Madin-Darby canine kidney epithelial cells, green fluorescent protein- tagged pleckstrin homology domain translocated from the plasma membrane to the cytoplasm in ... More
Initial and sustained calcium mobilizations in the parietal cell during stimulations with gastrin, inositol trisphosphate, phorbol ester and exogenous diacylglycerol.
AuthorsTsunoda Y, Takeda H, Asaka M, Nakagaki I, Sasaki S
JournalFEBS Lett
PubMed ID3130276
Electron probe X-ray microanalysis revealed that cytoplasmic Ca2+ concentration increased in the restricted apical cytoplasm during stimulation of isolated guinea pig parietal cells with gastrin. Furthermore, this study, using 45Ca2+, aequorin and fura-2, revealed the mechanism involved in intracellular Ca2+ shifts caused by gastrin and the involvements of inositol 1,4,5-trisphosphate ... More
Release of Ca2+ from individual plant vacuoles by both InsP3 and cyclic ADP-ribose.
AuthorsAllen GJ, Muir SR, Sanders D
JournalScience
PubMed ID7732384
Calcium mobilization from intracellular pools couples many stimuli to responses in plant cells. Cyclic adenosine 5'-diphosphoribose (cADPR), which interacts with a ryanodine receptor in certain animal cells, was shown to elicit calcium release at the vacuolar membrane of beet storage root. The vacuolar calcium release pathway showed similarities to cADPR-gated ... More
The effect of myo-inositol 1,4,5-trisphosphorothioate on Cl- current pattern and intracellular Ca2+ in the Xenopus laevis oocyte.
AuthorsFerguson JE, Potter B, Nuccitelli R
JournalBiochem Biophys Res Commun
PubMed ID1699536
Microinjection of myo-inositol 1,4,5-trisphosphate into voltage-clamped Xenopus laevis oocytes or the stimulation of the phosphatidylinositol cycle elicits a complex Ca2(+)-dependent Cl- current pattern. Microinjection of myo-inositol 1,3,4,5-tetrakisphosphate causes an immediate release of Ca2+, but elicits a different Cl- current pattern than myo-inositol 1,4,5-trisphosphate. We have studied the effects of myo-inositol ... More
Cytosolic calcium responses induced by photolytic release of 1,4,5-inositol trisphosphate in single human fibroblasts.
AuthorsPeres A, Racca C, Bertollini L, Sturani E
JournalBiochim Biophys Acta
PubMed ID2009312
We have used the whole cell technique to microinject human fibroblasts with either 1,4,5-inositol trisphosphate (InsP3) or 'caged' InsP3, in order to study the mechanisms of transmembrane signalling related to mitogenic stimulations. Cytosolic Ca2+ elevations in response to 1,4,5 InsP3 diffusing from the patch pipette were difficult to detect, while ... More
Influence of heparins on inositol 1,4,5-trisphosphate-induced calcium mobilization in permeabilized human platelets.
AuthorsRao GH, Fareed J, White JG
JournalBiochem Med Metab Biol
PubMed ID1883625
Heparin has been shown to prevent inositol 1,4,5-trisphosphate (IP3) binding to its receptor and to inhibit IP3-induced calcium mobilization in a variety of cells. Heparin added to whole blood at a concentration of 1 U/ml prevented thrombin-induced secretion of granule contents and irreversible aggregation of platelets. Heparin (2-15 kDa) had ... More
Ethanol-induced increases in [Ca2+]i and inositol (1,4,5) triphosphate in rat hepatocytes.
AuthorsReinlib L, Akinshola E, Potter JJ, Mezey E
JournalBiochem Biophys Res Commun
PubMed ID2268341
Rat hepatocytes were studied for [Ca2+]i with Fura-2 at the single cell level using a microfluorometer-imaging system which showed that both the number of cells elevating [Ca2+]i and the magnitude of [Ca2+]i increase were directly dependent upon ethanol concentration between 50 mM and 1 M. Peak [Ca2+]i increases ranged from ... More
Localized all-or-none calcium liberation by inositol trisphosphate.
AuthorsParker I, Ivorra I
JournalScience
PubMed ID2237441
Laser confocal microscopy was used to monitor calcium ion (Ca2+) liberation from highly localized (micrometer) regions of intact Xenopus oocytes in response to photo-released inositol 1,4,5-trisphosphate (InsP3). Local Ca2+ release varied in an all-or-none manner with increasing amount of InsP3, in contrast to signals recorded from larger areas, which grew ... More
Activation of sodium-proton exchange is not a prerequisite for Ca2+ mobilization and aggregation in human platelets.
AuthorsHunyady L, Sarkadi B, Cragoe EJ, Spät A, Gárdos G
JournalFEBS Lett
PubMed ID2826247
Recently it has been suggested [(1987) Nature 325, 456-458; (1987) FEBS Lett. 212, 123-126] that the activation of Na+/H+ exchange is a prerequisite for platelet aggregation and the development of the Ca2+ signal. As direct evidence for the role of the Na+/H+-exchange pathway the inhibition of the Ca2+ signal by ... More
TRPC1 and TRPC5 form a novel cation channel in mammalian brain.
AuthorsStrübing C, Krapivinsky G, Krapivinsky L, Clapham DE
JournalNeuron
PubMed ID11301024
TRP proteins are cation channels responding to receptor-dependent activation of phospholipase C. Mammalian (TRPC) channels can form hetero-oligomeric channels in vitro, but native TRPC channel complexes have not been identified to date. We demonstrate here that TRPC1 and TRPC5 are subunits of a heteromeric neuronal channel. Both TRPC proteins have ... More
Impact of mitochondrial Ca2+ cycling on pattern formation and stability.
AuthorsFalcke M, Hudson JL, Camacho P, Lechleiter JD
JournalBiophys J
PubMed ID10388738
Energization of mitochondria significantly alters the pattern of Ca2+ wave activity mediated by activation of the inositol (1,4,5) trisphosphate (IP3) receptor (IP3R) in Xenopus oocytes. The number of pulsatile foci is reduced and spiral Ca2+ waves are no longer observed. Rather, target patterns of Ca2+ release predominate, and when fragmented, ... More
Ca(2+)-induced Ca2+ release in sea urchin egg homogenates: modulation by cyclic ADP-ribose.
AuthorsGalione A, Lee HC, Busa WB
JournalScience
PubMed ID1909457
Calcium-induced calcium release (CICR) may function widely in calcium-mediated cell signaling, but has been most thoroughly characterized in muscle cells. In a homogenate of sea urchin eggs, which display transients in the intracellular free calcium concentration ([Ca2+]i) during fertilization and anaphase, addition of Ca2+ triggered CICR. Ca2+ release was also ... More
Beyond receptors: multiple second-messenger systems in brain.
AuthorsWorley PF, Baraban JM, Snyder SH
JournalAnn Neurol
PubMed ID3037986
Second-messenger systems play a major role in mediating neurotransmitter actions. In recent years our understanding of the organization and function of two prominent second-messenger systems has progressed rapidly--the adenylate cyclase and phosphoinositide systems. Guanosine triphosphate-binding proteins, which are especially abundant in brain, couple transmitter receptors to the key second-messenger generating ... More
Protein kinase C differentially inhibits muscarinic receptor operated Ca2+ release and entry in human salivary cells.
AuthorsHe XJ, Wu XZ, Baum BJ
JournalBiochem Biophys Res Commun
PubMed ID3377766
We investigated the effects of phorbol myristate acetate on muscarinic receptor-induced Ca2+ release from intracellular stores and extracellular entry in a human salivary duct cell line, HSG-PA. Phorbol myristate acetate (approximately 10(-7) M) blocked both Ca2+ release and Ca2+ entry induced by the muscarinic agonist carbachol. This blockade was the ... More
Unique inactivation properties of NAADP-sensitive Ca2+ release.
AuthorsGenazzani AA, Empson RM, Galione A
JournalJ Biol Chem
PubMed ID8662773
Ca2+ mobilization from intracellular stores constitutes an important mechanism for generating cytoplasmic Ca2+ signals. Inositol trisphosphate (InsP3) and ryanodine receptors are the two families of intracellular Ca2+ release channels that have been identified, which may be regulated by separate intracellular messengers, InsP3, and cyclic adenosine 5'-diphosphate ribose, respectively. A third ... More
Calcium and inositolphosphates in the activation of T cell-mediated cytotoxicity.
AuthorsTreves S, Di Virgilio F, Cerundolo V, Zanovello P, Collavo D, Pozzan T
JournalJ Exp Med
PubMed ID3036996
Reports from a number of laboratories have shown that mAbs against the T3-Ti receptor complex cause an increase in cytosolic-free Ca2+ [( Ca2+]i) and the hydrolysis of phosphatidylinositolbisphosphate (PIP2) in CTLs. In the present report we show that activation of CTLs by their specific targets causes: (a) release of Ca2+ ... More
Effects of inositol trisphosphate on calcium mobilization in bone cells.
AuthorsFalsafi R, Tatakis DN, Hagel-Bradway S, Dziak R
JournalCalcif Tissue Int
PubMed ID1782574
The effect of inositol 1,4,5 trisphosphate (IP3) on calcium mobilization was studied in human osteosarcoma lines, Saos-2 and G292, as well as isolated rat osteoblastic and osteoclastic cells. Cells were permeabilized with saponin and calcium mobilization was studied with the fluorescent dye, fura-2 in a recording spectrofluorometer. IP3 (10 microM) ... More
Quantal Ca2+ mobilization stimulated by inositol 1,4,5-trisphosphate in permeabilized hepatocytes.
AuthorsOldershaw KA, Nunn DL, Taylor CW
JournalBiochem J
PubMed ID1898359
In several cell types, including hepatocytes, submaximal concentrations of Ins(1,4,5)P3 stimulate an initial rapid mobilization of intracellular Ca2+ stores that is followed by either no further Ca2+ release or very much slower release. Further additions of Ins(1,4,5)P3 then evoke further Ca2+ mobilization. Such 'incremental' responses [Meyer & Stryer (1990) Proc. ... More
Diffusion across the nuclear envelope inhibited by depletion of the nuclear Ca2+ store.
AuthorsStehno-Bittel L, Perez-Terzic C, Clapham DE
JournalScience
PubMed ID8525380
Intact, isolated nuclei and a nuclear membrane (ghost) preparation were used to study regulation of the movement of small molecules across the Xenopus laevis oocyte nuclear membrane. In contrast to models of the nuclear pore complex, which assume passive bidirectional diffusion of molecules less than 70 kilodaltons, diffusion of intermediate-sized ... More