Invitrogen™

iBlot™ 2 and iBind™ Western Starter Kit

Catalog number:  IB2200S

 Related applications:

Protein Electrophoresis & Western Blotting

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Description

The iBlot 2 and iBind Western Starter Kit includes the iBlot 2 Gel Transfer Device, the iBind Western Device, and all of the consumables and reagents necessary to get started using both devices for fast, efficient transfer and western processing.

Contents of the iBlot 2 and iBind Western Starter Kit:
iBlot 2 Gel Transfer Device
iBlot 2 Transfer Stacks, nitrocellulose, regular size
iBind Western Device
iBind Cards
iBind Solution Kit
SuperSignal West Pico Plus Chemiluminescent Substrate

iBlot 2 Gel Transfer Device
The iBlot 2 Gel Transfer Device performs western blotting transfer simply, efficiently, and reliably, within seven minutes without the need for liquid buffers. The iBlot 2 system is compatible with both polyvinylidene difluoride (PVDF) and nitrocellulose membranes, and has comparable performance to traditional wet transfer methods in a fraction of the time. The iBlot 2 Gel Transfer Device is an integral part of the iBlot 2 Dry Blotting system, which consists of the transfer device and consumable transfer stacks that contain the required buffers and transfer membrane.

iBind Western Device
The iBind Western Device is an automated western-processing device that performs every step in the western protocol from blocking to washes to antibody incubations via sequential lateral flow (SLF). SLF allows the timely release and flow of solutions and antibodies to the membrane without need of an external power source. Each solution is released from the iBind device wells to an iBind Card via SLF; the solutions are then wicked towards the stack region of the card. The glass fiber matrix of the card allows for homogenous and consistent flow of the solutions to the membrane, increasing the antigen-antibody interaction. The iBind western detection procedure minimizes hands-on time and is highly reproducible and compatible with all western protocols. Set up time is fast: in less than 3 hours you are ready for the detection step.

Learn more about the iBlot 2 system ›
Learn more about the iBind western device ›

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Mode of Transfer: Dry
Gel Compatibility: Bis-Tris Gels, E-PAGE™ Gels, Midi, Mini, Tricine Gels, Tris-Acetate Gels, Tris-Glycine Gels
Product Line: iBlot2™
Product Size: 1 kit
Shipping Condition: Approved for shipment at Room Temperature and wet ice

Contents & storage

• iBlot 2 Gel Transfer Device
• iBlot 2 Transfer Stacks, nitrocellulose, regular size
• iBind Western Device
• iBind Cards
• iBind Solution Kit
• SuperSignal West Pico Plus Chemiluminescent Substrate

Documents

Manuals & protocols