iBlot 2 Transfer Stacks are used to transfer proteins using the iBlot 2 Gel Transfer Device. They are consumable stacks with an integrated pre-activated PVDF transfer membrane for dry blotting of proteins. Each iBlot 2 Transfer Stack contains a copper-coated electrode and appropriate cathode and anode buffers in the gel matrix to allow fast, reliable transfer of proteins. This mini-size stack (8 cm x 8 cm) can be used with one mini-size gel.
PVDF membrane The PVDF membrane (0.2-µm pore size, low fluorescence) in this stack has higher binding capacity than nitrocellulose. It is pre-activated and ready for use without any pretreatment with alcohols. The membrane is compatible with commonly used detection methods such as staining, immunodetection, fluorescence, and radiolabeling. The proteins bind to the membrane due to hydrophobic interactions. The protein-binding capacity is 240 µg/cm2.