Invitrogen™

iBlot™ 3 Starter Kit, PVDF

Catalog number: IB31002S
Have Questions?
Invitrogen™

iBlot™ 3 Starter Kit, PVDF

Catalog number: IB31002S
Have Questions?
Catalog Number
IB31002S
Unit Size
1 kit
Price (USD)
Quantity
Catalog Number
Unit Size
1 kit with warranty
Price (USD)
Quantity
Catalog NumberUnit SizePrice (USD)Quantity
IB31002S1 kit
A567291 kit with warranty
Product Overview
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The iBlot 3 Starter Kit, PVDF, includes the iBlot 3 Western Blot Transfer Device and two boxes of iBlot 3 Transfer Stacks, Midi, PVDF—everything needed to perform a rapid dry-transfer of proteins from a gel to a membrane.

iBlot 3 Western Blot Transfer Device
The iBlot 3 Western Blot Transfer Device is a dry transfer device that performs western blotting transfer efficiently and reliably in as few as three minutes and without the need for liquid buffers. The iBlot 3 system is compatible with both nitrocellulose and polyvinylidene difluoride (PVDF) membranes and offers equivalent or better performance compared with traditional wet-transfer methods in a fraction of the time. The iBlot 3 Western Blot Transfer Device is an integral part of the iBlot dry blotting system, which consists of the transfer device and consumable transfer stacks that contain the required buffers and transfer membrane (nitrocellulose or PVDF).

iBlot 3 Western Blot Transfer Device features include:
• Complete protein transfer in as few as three minutes
• High detection sensitivity and even, consistent protein transfer
• Two independently controlled transfer stations capable of transferring up to two midi or four mini blots
• Built-in adjustable cooling to ensure consistent transfer temperature
• Pre-programed transfer methods optimized for low, high, and broad molecular weight ranges
• Ability to create custom programs
• A simple, user-friendly interface

How the iBlot dry blotting system works
Buffer ion reservoirs are incorporated into the gel matrix of transfer stacks instead of buffer tanks or soaked filter papers. The high density of ions in the gel matrix enables rapid protein transfer. During blotting, the copper anode does not generate oxygen gas as a result of water electrolysis, reducing blot distortion (conventional protein transfer techniques, including wet, semi-wet, and semi-dry, use inert electrodes that generate oxygen).

With the iBlot dry blotting system, transfer time is reduced by the shortened distance between electrodes, high field strength, and high current. Trapped air bubbles, often created during the manual preparation of the blotting sandwich layers, are easy to avoid due to our unique de-bubbling design that promotes even and complete transfer. With the iBlot 3 system, there is no need to prepare buffers or clean up after blotting. The total preparation and run time is normally less than ten minutes per blot.

Specifications

For Use With (Application)
Western Blot
For Use With (Equipment)
iBlot 3 Transfer Stacks
Gel Compatibility
Mini- or Midi-sized: Bis-Tris Gels, Tricine Gels, Tris-Acetate Gels, and Tris-Glycine Gels
Membrane Compatibility
Nitrocellulose, PVDF
Mode of Transfer
Dry
Product Line
iBlot 3
Running Time
∼3 to 10 min.
Throughput
2 Midi or 4 Mini Gels
Type
Starter kit
Green Features
Less waste, Sustainable packaging, Sustainable disposal
Shipping Condition
Approved for shipment at Room Temperature or on Wet Ice
Warranty
Standard, 1-year warranty included

Contents & Storage

• iBlot 3 Gel Transfer Device
• Western Blot Roller
• iBlot 3 Transfer Stacks, midi, PVDF (2 boxes, 20 stacks)

Store at room temperature.

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