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Thermo Scientific™
Biochemical Identification System (O.B.I.S.) Mono
Catalog number: ID0600M
Thermo Scientific™
Biochemical Identification System (O.B.I.S.) Mono
Catalog number: ID0600M
Rapidly differentiate Listeria monocytogenes from other Listeria species with Thermo Scientific™ Oxoid™ Biochemical Identification System (O.B.I.S.) mono. This rapid colorimetric test delivers simple and rapid results for the determination of D-alanyl aminopeptidase (DALAase). It has been designed for the differentiation of presumptive Listeria species that have been isolated from selective media and plated onto a secondary medium for further biochemical testing.
Identification of Listeria monocytogenes relies on the lack of the enzyme D-alanyl aminopeptidase.1,2,3
| Detectable Analytes | L. monocytogenes |
| Quantity | 60 Tests/Kit |
| Unit Size | Each |
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Listeria monocytogenes is the most common pathogenic Listeria spp. and has been shown to be pathogenic to both humans and animals.
Use O.B.I.S. mono to rapidly differentiate Listeria monocytogenes from other Listeria spp., reducing the need for a full biochemical identification of suspect colonies.
- Simple—easy to implement
- Rapid—results in 6 minutes of testing suspect colonies
- Non-carcinogenic substrate
We have developed a new system for aminopeptidase testing4 using a non-carcinogenic substrate in response to health concerns associated with amino acid conjugates of ß naphthylamine5,6 as these are potent carcinogens.
Not all products are available for sale in all territories. Please inquire.
Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.
General References:
- Kämpfer, P., Böttcher, S., Dott, W., Rüden, H. (1991) Physiological characterization and identification of Listeria species. Zentralblatt für Bakteriologie 275, 423 435.
- Kämpfer, P. (1992) Differentiation of Corynebacterium spp., Listeria spp., and related organisms by fluorogenic substrates. Journal of Clinical Microbiology 30, 1067 1071.
- Clark, A.G., McLauchlin, J. (1997) Simple color tests based on an alanyl peptidase reaction which differentiate Listeria monocytogenes from other Listeria species. Journal of Clinical Microbiology 35, 2155-2156
- Morgan, J. W. (1987) Evaluation of a rapid method for the determination of L pyrrolidonyl-ß-naphthylamide hydrolysis. Laboratory Medicine 18, 682-683.
- Bascomb, S. and Manafi, M. (1998) Use of enzyme tests in characterization and identification of aerobic and facultatively anaerobic Gram-positive cocci. Clinical Microbiology Reviews 11, 318-340.
- Anonymous (2000) 2-Naphthylamine. In Croner's Hazardous Substances: Carcinogens Guide. pp. 2-291. Kingston-upon-Thames: Croner.CCH Group Limited.
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