Vanquish™ Flex Quaternary UHPLC System, Integrated biocompatible UHPLC system with quaternary pump - FAQs

What maintains temperature control and method reproducibility in the Vanquish Flex UHPLC System?

Column Compartment H provides still air/forced air modes, active preheating, and broad temperature ranges for both the column and samples, supporting consistent retention and peak shape.

How many samples can we queue on a Vanquish Flex UHPLC System? Can it run all weekend?

The autosampler holds 4 sample plates by default and can scale to 8,832 samples (23 plates) with the Vanquish Sample Hub module for extended unattended operation.

What are the performance limits of a Vanquish Flex UHPLC System?

The Vanquish Flex UHPLC System delivers up to 1000 bar (15,000 psi) and 8 mL/min, with industry-leading flow/gradient precision via SmartFlow technology.

Should I choose a binary or quaternary Vanquish Flex UHPLC System for method development and routine runs?

Choose quaternary for straightforward low-pressure mixing across 4 solvent channels; choose binary for high-pressure mixing of two solvents out of up to 6 channels when you want fast, precise gradients and solvent flexibility.

What’s special about the Vanquish Neo UHPLC System’s autosampler design for proteomics?

The split-loop autosampler minimizes sample dispersion, provides thorough washing of fluidics and columns, and decreases maintenance while enabling fast loading/equilibration—ideal for high-sensitivity and high-throughput methods.

How does the Vanquish Neo UHPLC System ensure reproducible retention times across such a wide flow rate range?

ProFlow XR active flow control is available from 1 nL/min to 100 µL/min and provides accurate and precise gradient delivery for stable retention time across long and ultrashort runs.

We have legacy HPLC methods. What tools are available to simplify transferring our methods to Vanquish HPLC systems?

Thermo Scientific Vanquish HPLC systems include tools to replicate method behavior, such as tunable gradient delay volume and multiple column heating modes that match existing conditions. They are expressly designed to simplify method transfer from other instruments.

I’m trying to choose the right Vanquish (U)HPLC system. How do Vanquish Core HPLC, Vanquish Flex UHPLC and Vanquish Neo UHPLC differ?

Think of the Thermo Scientific Vanquish Core HPLC for routine analytical work with straightforward gradients, the Thermo Scientific Vanquish Flex UHPLC for high-throughput and flexible method development (binary or quaternary mixing), and the Thermo Scientific Vanquish Neo UHPLC for low-flow LCMS (nano/capillary/micro). Vanquish Core HPLC goes to 700 bar (10,000 psi) with flows up to 10 mL/min; Vanquish Flex UHPLC to 1000 bar (15,000 psi) with up to 8 mL/min; Vanquish Neo UHPLC to 1500 bar (22,000 psi) and 1 nL/min–100 µL/min for ultrasensitive LCMS analysis.

What are the main differences between the iCAP PRO, iCAP PRO X, iCAP PRO XP, and iCAP PRO XPS models?

The main differences among the Thermo Scientific iCAP PRO models are based on their specific features and intended applications:

• iCAP PRO: For routine laboratory analysis with fast start-up, easy-to-use software, and robust performance. It simplifies analysis in laboratories performing standardized analysis for a low number of samples.
• iCAP PRO X: For high-matrix trace elemental analysis with sensitive multi-element detection, ideal for challenging samples. It helps laboratories with moderate throughput and established methods to improve performance and reliability.
• iCAP PRO XP: Flexibility for method optimization and achieves advanced performance using an enhanced ultraviolet (eUV) analysis mode, which measures the UV region of the spectrum with greater sensitivity. Fast data acquisition times allow for high throughput labs to stay competitive.
• iCAP PRO XPS: Exceptional throughput and versatility, meeting specific regulatory requirements with high-speed analysis and excellent stability. With short integration times and high flexibility, this system is appropriate for testing labs that require the high throughput and performance.