Pinpoint™ Software

Thermo Scientific™  Related applications: Industrial Mass Spectrometry

Facilitate the transition from proteomic discovery experiments to verification of putative biomarkers and general quantitative proteomics with Thermo Scientific™ Pinpoint™ software. Pinpoint software simplifies development and refinement of targeted quantitative methods. It uses data from previous discovery experiments to predict proteotypic peptides and determine the best SRM transitions. It also provides tools for evaluating preliminary data so that only peptides showing the best response are retained in the final intelligent SRM (iSRM) method.

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Description

Simplifies SRM Assay Development

  • Imports and processes panels of targeted proteins and peptides selected from discovery experiments
  • Automates searching of user-created and public (e.g. Peptide Atlas) MS/MS spectral libraries, or in-silico prediction of target peptides for hypothesis-driven experiments
  • Automates selection of the most abundant precursor and product ions, with highly accurate, automated determination of optimal collision energies for SRM transitions
  • Provides flexible method design that can integrate high-resolution precursor selection, timed acquisition, and full MS/MS scans (QED) into SRM assays
  • Matches experimental and library spectra, providing correlation analysis with probability scoring for high-confidence targeted peptide verification
  • Integrates discovery results with relative and absolute quantitation results in customizable reports
  • Easily exports results for use in refining the method
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Manuals & protocols