iPrep™ ChargeSwitch® Forensic Kit

Catalog number: IS10002

Invitrogen™  Related applications: Automated Nucleic Acid Purification | DNA Extraction | Sample Prep⁄Extraction for Human Identification

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Description

• Flexibility in starting sample type, for quick and easy processing of different samples simultaneously
• High sensitivity with samples containing very low amounts of DNA
• Fast automated extraction time of 20 minutes
• Standardized procedures minimize human error; preset protocols and prefilled cartridges eliminate variation

The iPrep ChargeSwitch® Forensic Kit offers a single protocol for efficiently isolating DNA from all kinds of forensic samples (Table 1), even if
the DNA copy number is low or the DNA is partially degraded.

Designed specifically for use with the iPrep™ Purification Instrument, the iPrep™ ChargeSwitch® Forensic Kit takes advantage of the powerful ChargeSwitch® technology, a major advance in DNA purification. This technology is based on a unique, ionizable nucleic acid-binding ligand whose charge can be switched based on the pH of the surrounding medium (Figure 1). Purification is achieved through a simple three-step procedure in aqueous buffers, and avoids the use of guanidine, ethanol, and other troublesome reagents.

Contents and Storage:
The iPrep™ ChargeSwitch® Forensic Kit provides all reagents (supplied in prefilled cartridges), tubes, and tips needed for isolating DNA from forensic cell samples. Reagent cartridges can be stored at room temperature; store lysis buffer and proteinase K at 4°C.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Sample Type (General): Forensic Samples
Isolation Technology: ChargeSwitch® Chemistry
Downstream Application: PCR, Forensic Testing, Sequencing, Southern Blotting, Cloning
High Throughput Compatibility: Not High Throughput-Compatible (Manual)
Product Size: 52 preps

Documents

Manuals & protocols