Anza™ 97 Bsp143I
This product will be discontinued on September 30th, 2024. Alternative product: FD0784
Invitrogen™
Anza™ 97 Bsp143I
5' ↓ G A T C 3'3' C T A G ↑ 5'Invitrogen™ Anza™ 97 Bsp143I is a restriction enzymeRead more
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Catalog number IVGN0976
Price (USD)/ 800 units
117.00
-
Add to cart
Price (USD)/ 800 units
117.00
Add to cart
Anza™ 97 Bsp143I
Catalog numberIVGN0976
Price (USD)/ 800 units
117.00
-
Add to cart
5' ↓ G    A    T    C    3'
3'    C    T    A    G ↑ 5'


Invitrogen™ Anza™ 97 Bsp143I is a restriction enzyme that cuts DNA at this recognition site: ^GATC, completely digesting the DNA in 15 minutes at 37°C. Isoschizomers include: BfuCI, BssMI, BstMBI, DpnII, Kzo9I, MboI, NdeII, Sau3AI.

For superior convenience, a single buffer and protocol are used with all Anza™ restriction enzymes. The single Anza buffer allows multiple restriction enzymes to be used simultaneously in one reaction. This helps eliminate the need for sequential digests and the DNA clean-up necessary when changing buffers. Additionally, the Anza buffer and restriction enzymes have been designed for flexibility in digestion times. Digestions of up to 16 hours can be carried out without star activity.

Convenient—all Anza enzymes are 100% functional in the single Anza buffer
Fast—complete digestion in 15 minutes
Flexible—overnight digestion without star activity
Simple—a single digestion protocol for all DNA types
Complete system—incorporates Anza DNA Modifying Enzymes for a complete cloning experience

Anza DNA Modifying Enzymes include the Anza T4 DNA Ligase Master Mix, Anza Alkaline Phosphatase Kit, Anza T4 PNK Kit, Anza DNA Blunt End Kit, and Anza DNA End Repair Kit. Use these enzymes for a complete cloning workflow.

Anza Red Buffer, which contains a density gradient and tracking dyes, is supplied for added convenience. Digestion reactions are as efficient in the Anza Red Buffer as in the Anza Buffer. Use of the Anza Red Buffer helps reduce pipetting steps when preparing multiple digestion reactions and a subsequent step includes agarose gel electrophoresis. For applications that require product analysis by fluorescence excitation, the colorless Anza Buffer without the tracking dyes is recommended.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Shipping ConditionApproved for shipment on Wet or Dry Ice
Methylation SensitivityCpG Methylation-Sensitive, Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive
EnzymeBsp143I
Compatible BufferAnza™ 10X Red Buffer, Anza™ 10X Buffer
Sensitive to Heat InactivationYes
Optimal Reaction Temperature37°C
Type IIS RENo
Quantity800 units
Product TypeRestriction Enzyme Cloning System
Research CategoryTraditional Cloning
Product LineAnza™
Unit Size800 units
Contents & Storage
80 μL Anza 97 Bsp143I
500 μL Anza Buffer (10X)
500 μL Anza Red Buffer (10X)

Store at -20°C.