Invitrogen™

Anza™ DNA Blunt End Kit

Catalog number:  IVGN2404

Related applications:

Restriction Enzyme Cloning

Back to top

Description

The Invitrogen™ Anza™ DNA Blunt End Kit is used to convert DNA with cohesive ends to DNA with blunt ends for use in blunt-end ligation reactions. The included Anza™ DNA Blunting Enzyme Mix contains T4 DNA polymerase and Klenow Fragment, while the Anza™ 10X Blunting Buffer contains dNTPs to facilitate the synthesis of blunt ends. Use this kit when the desired vector does not contain a recognition site that would produce blunt ends.

Features:
• Fast 15-minute, room-temperature protocol
• DNA digested with Anza™ restriction enzymes can be used directly in the Anza Blunt End protocol following heat inactivation

The Anza DNA Blunt End Kit is part of the Anza™ Restriction Enzyme Cloning System, unifying the traditional cloning processes. Other Anza DNA Modifying Enzymes include the Anza T4 DNA Ligase Master Mix, Anza T4 PNK Kit, and Anza DNA End Repair Kit.

For more information on the Anza system, visit www.thermofisher.com/anza.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Product Line: Anza™
Shipping Condition: Approved for shipment on Wet or Dry Ice
Enzyme: T4 DNA polymerase, Klenow Fragment

Contents & storage

100 µL Anza Blunting Enzyme Mix
200 µL Anza 10X Blunting Buffer

Store at -5 to -30°C.