Anza™ 5-Pack Starter Kit

Catalog number:  IVGN3004

 Related applications:

Restriction Enzyme Cloning

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The Invitrogen™ Anza™ 5-Pack Starter Kit includes the 5 common restriction enzymes, 2 DNA modifying enzymes, and buffers listed below:

• Anza™ 11 EcoRI
• Anza™ 5 BamHI
• Anza™ 1 NotI
• Anza™ 16 HindIII
• Anza™ 12 XbaI
• Anza™ T4 DNA Ligase Master Mix
• Anza™ Alkaline Phosphatase
• Anza™ Buffer (10X)
• Anza™ Red Buffer (10X)

For a kit with 10 enzymes, see the Anza™ 10-Pack Starter Kit.

The Anza™ Restriction Enzyme Cloning System employs a single buffer and protocol for all Anza restriction enzymes. The single Anza buffer allows multiple restriction enzymes to be used simultaneously in one reaction. This eliminates the need for sequential digests and the DNA clean-up necessary when changing buffers. Additionally, the Anza buffer and restriction enzymes have been designed for flexibility in digestion times. Digestions of up to 16 hours can be carried out without star activity.

Convenient—all Anza enzymes are 100% functional in the single Anza buffer
Fast—complete digestion in 15 minutes
Flexible—overnight digestion without star activity
Simple—a single digestion protocol for all DNA types
Complete system—incorporates Anza DNA Modifying Enzymes for a complete cloning experience

Anza Red Buffer, which contains a density gradient and tracking dyes, is supplied for added convenience. Digestion reactions are as efficient in the Anza Red Buffer as in the Anza Buffer. Use of the Anza Red Buffer helps reduce pipetting steps when preparing multiple digestion reactions and a subsequent step includes agarose gel electrophoresis. For applications that require product analysis by fluorescence excitation, the colorless Anza Buffer without the tracking dyes is recommended.

The Anza 5-pk Starter Kit is a subset of the Anza Restriction Enzyme Cloning System, unifying the traditional cloning processes. For more information on the system, visit
For Research Use Only. Not for use in diagnostic procedures.


Compatible Buffer: Anza™ 10x Buffer, Anza™ 10x Red Buffer
Enzyme: Alkaline Phosphatase, BamHI, EcoRI, HindIII, NotI, T4 DNA Ligase, XbaI
Methylation Sensitivity: Methylation sensitivities varies by enzyme. Check enzyme insert for specifics.
Product Line: Anza™
Product Size: 1 kit
Shipping Condition: Approved for shipment on Wet or Dry Ice

Contents & storage

• 20 µL each of Anza 11 EcoRI, Anza 5 BamHI, Anza 1 NotI, Anza 16 HindIII, Anza 12 XbaI
• 100 µL Anza T4 DNA Ligase Master Mix
• 20 µL Anza Alkaline Phosphatase
• 500 µL Anza Buffer (10X)
• 500 µL Anza Red Buffer (10X)

Store at -5 to -30°C.


Manuals & protocols