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LB Agar plate with 50μg/mL Kanamycin
Catalog number: J60540.EQF
20 Plates, Each
Thermo Scientific Chemicals
LB Agar plate with 50μg/mL Kanamycin
Catalog number: J60540.EQF
20 Plates, Each
Quantity
Catalog number: J60540.EQF
also known as J60540-=F
Price (USD)
Specifications
Specification SheetAppearance (Color)
Yellow to light grey
Composition
16 gm/ L Agar-agar melted in LB medium and 50 µg/ml Kanamycin
Form
Soft gel
Comment
Agar-agar is mixed with LB medium, autoclaved at 15 PSI (121?C) for 60 minutes, cooled to 52 to 50?C, added antibiotic, poured onto petri-dishes, polymerized and dry under sterile aseptic condition.
Description
LB Agar plate with 50μg/ml kanamycin is suitable for selective cultivation of E.coli strains that contain plasmids conferring kanamycin resistance. Further, it is used for DNA plasmid production or production of recombinant proteins.
This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.
General Description
• LB agar plate is a nutrient-rich media frequently used to culture bacteria in the lab, including Escherichia coli
Application
• LB agar plate is used to culture bacteria in microbiology research. These plates are commonly used during cloning experiments to amplify antibiotic-resistant, competent bacteria
• The addition of agar to LB medium helps form a gel that allows bacteria to grow. The bacteria cannot digest the agar but can obtain the needed nutrients to grow from the LB medium
• The addition of ampicillin (100 μg/mL) to these plates allows selection of the bacteria that can grow in the presence of ampicillin which is conferred by a plasmid carrying the antibiotic resistance gene
RUO – Research Use Only