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Ponceau S, Electrophoresis Grade, Thermo Scientific Chemicals
Protein stain for electrophoresis
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Catalog number J60744.18
also known as J60744-18
Price (USD)/ Each
54.65
Online exclusive
64.00 Save 9.35 (15%)
-
Quantity:
50 g
Price (USD)/ Each
54.65
Online exclusive
64.00 Save 9.35 (15%)
Ponceau S, Electrophoresis Grade, Thermo Scientific Chemicals
Catalog numberJ60744.18
Price (USD)/ Each
54.65
Online exclusive
64.00 Save 9.35 (15%)
-
Chemical Identifiers
IUPAC Nametetrasodium (4Z)-3-oxo-4-(2-{2-sulfonato-5-[2-(4-sulfonatophenyl)diazen-1-yl]phenyl}hydrazin-1-ylidene)-3,4-dihydronaphthalene-2,7-disulfonate
Molecular FormulaC22H12N4Na4O13S4
InChI KeyLZYGEJPERQQCIY-STNZDNLRSA-J
SMILES[Na+].[Na+].[Na+].[Na+].[O-]S(=O)(=O)C1=CC=C(C=C1)N=NC1=CC=C(C(N\N=C2/C(=O)C(=CC3=CC(=CC=C23)S([O-])(=O)=O)S([O-])(=O)=O)=C1)S([O-])(=O)=O
Molecular Weight (g/mol)760.55
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Specifications
Specification Sheet
Specification SheetFormPowder
Loss on Drying≤5.0%
Assay (Dye)Dye Content: ≥80.0%
Solution TestConforms (0.1% Water)
Appearance (Color)Red-brown to dark brown
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Ponceau S dye is used to make a stain for rapid reversible staining of protein bands on nitrocellulose or PVDF membranes (Western blots) and also for staining protein on cellulose acetate membranes. It is also suitable for electrophoresis, azo dye for reversible protein staining. This stain can also be used as a fiducial marker in mass spectrometric imaging.
This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.
Applications
Ponceau S dye is used to make a stain for rapid reversible staining of protein bands on nitrocellulose or PVDF membranes (Western blots) and also for staining protein on cellulose acetate membranes. It is also suitable for electrophoresis, azo dye for reversible protein staining. This stain can also be used as a fiducial marker in mass spectrometric imaging.
Solubility
Soluble in water (10 g/L) at 20°C. Insoluble in ether.
Notes
Store in cool place. Keep container tightly closed in a dry and well-ventilated place. Keep away fom strong oxidizing agents.
Ponceau S dye is used to make a stain for rapid reversible staining of protein bands on nitrocellulose or PVDF membranes (Western blots) and also for staining protein on cellulose acetate membranes. It is also suitable for electrophoresis, azo dye for reversible protein staining. This stain can also be used as a fiducial marker in mass spectrometric imaging.
Solubility
Soluble in water (10 g/L) at 20°C. Insoluble in ether.
Notes
Store in cool place. Keep container tightly closed in a dry and well-ventilated place. Keep away fom strong oxidizing agents.
RUO – Research Use Only
General References:
- RH Aebersold.; J Leavitt.; RA Saavedra. Internal amino acid sequence analysis of proteins separated by one-or two-dimensional gel electrophoresis after in situ protease digestion on nitrocellulose. PNAS. 1987, 84,(20), 6970-6974.
- MA Paz, R Flückiger, A Boak, HM Kagan. Specific detection of quinoproteins by redox-cycling staining. The Journal of Biological Chemistry.1991, 266, 689-692.