Detergent, equivalent to Nonidet P-40. For solubilizing membrane proteins, IGEPAL™ CA-630 is used as a detergent in paper industry, hand cleaners, metal cleaners. It is suitable for solubilization, isolation and purification of membrane protein complexes and in two-dimensional gel electrophoresis.
This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.
General Description
- IGEPAL|r CA-630 is a nonionic and non-denaturing detergent similar to Nonidet P-40
Application
- IGEPAL|r CA-630 is mainly used for the solubilization, isolation, and purification of membrane protein complexes and in two-dimensional gel electrophoresis
- IGEPAL|r CA-630 can be used in the composition of homogenizing buffers during western blotting technique in mouse muscle homogenates
- It has been used in X-gal staining to study the lacZ expression
- IGEPAL|r CA-630 is also a component of the cell lysis buffer in order to prepare RT-qPCR-ready cell lysates
RUO – Research Use Only
Bertin, F. R.; Lemarié, C. A.; Robins, R. S.; Blostein, M. D. Growth arrest-specific 6 regulates thrombin-induced expression of vascular cell adhesion molecule-1 through forkhead box O1 in endothelial cells. J. Thromb. Haemost. 2015, 13 (12), 2260-2272.
Blackford, A. N.; Nieminuszczy, J.; Schwab, R. A.; Galanty, Y.; Jackson, S. P.; Niedzwiedz, W. TopBP1 Interacts with BLM to Maintain Genome Stability but Is Dispensable for Preventing BLM Degradation. Mol. Cell 2015, 57 (6), 1133-1141.