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Search Thermo Fisher Scientific
BSA blocking buffer have a high affinity for proteins. A variety of blocking buffers ranging from milk or normal serum to highly purified proteins have been used to block free sites on a membrane. It is a good blocking agent that is easily prepared in the lab. It can be used in a range from 0.3% to 5%. It is also useful for saturating excess protein-binding sites on the membranes and microplates in immunoassays.
This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.
General Description
• BSA blocking buffer contains a serum albumin protein isolated from a cow with a high affinity for proteins
Application
• BSA (bovine serum albumin) blocking buffer is used as a blocking reagent ideal for saturating excess protein-binding sites on membranes and microplates for Western blotting and ELISA applications
• This buffer can be used in a range from 0.3% to 5% dissolved in PBS solution
Literature References
Kalwa, H.; Storch, U.; Demleitner, J.; Fiedler, S.; Mayer, T.; Kannler, M.; Fahlbusch, M.; Barth, H.; Smrcka, A.; Hildebrandt, F.; Gudermann, T.; Dietrich, A. Phospholipase C Epsilon (PLCepsilon) Induced TRPC6 Activation: A Common but Redundant Mechanism in Primary Podocytes. J. Cell. Physiol. 2015, 230 (6), 1389-1399.
Jeong, M. S.; Ahn, D. R. A microwell plate-based multiplex immunoassay for simultaneous quantitation of antibodies to infectious viruses. Analyst 2015, 140 (6), 1995-2000.