RBC lysis buffer for mouse

Name: RBC lysis buffer for mouse
SKU: J62150.AK

For lysis of mouse red blood cells

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Quantity:

250mL

500mL

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Catalog number J62150.AK

also known as J62150-AK

Price (TWD)

Quantity:

250mL

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Specifications

For Use With (Application)For lysis of mouse red blood cells

FormatLiquid

Quantity250mL

Recommended StorageKeep cold

Product TypeCell Lysis Reagent

RBC Lysis Buffer has been reported for use in flow cytometric analysis and cell culture. Red Blood Cell Lysing Buffer has been developed for use in hybridoma protocols to remove red blood cells from mouse splenocyte suspensions before fusion. It is also useful in systems where it may be desirable to remove red blood cells from cell suspensions, such as whole blood.

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

RUO – Research Use Only

Contents & Storage

2° to 8°C

Frequently asked questions (FAQs)

What is the procedure for use of RBC lysis buffer for mouse (Cat. No. J62150.AP, J62150.AK)?

We recommend the following procedure:
1. Dilute 1 volume of cell suspension with 10 volumes of RBC lysis buffer for mouse.
2. Vortex for 5 sec and incubate for 10 min at room temperature. If the sample is not transparent red after 10 min, incubate for another 2 min. Stop reaction by adding 20-30 mL of 1X PBS.
3. Centrifuge at 300xg for 10 min at 4 degrees C. Aspirate supernatant. White blood cells are present in the pellet.
4. Repeat the lysis process if red blood cells are still present in the pellet.
5. Resuspend the cell pellet in an appropriate buffer and proceed for further applications.

How do I use the RBC lysis buffer for mouse (Cat. No. J62150.AP, J62150.AK)?

This buffer is able to lyse mouse RBCs. It contains ammonium chloride that lyses the RBC and leaves the WBCs intact. The resulting WBCs can be used for staining or nucleic acid isolation.

What are the concentrations of the components of the RBC lysis buffer for mouse (Cat. No. J62150.AP, J62150.AK)?

RBC lysis buffer for mouse contains 10 mM Tris-HCl, 150 mM ammonium Chloride at pH 7.2-7.4.