Glycerol is used both in sample preparation and gel formation for polyacrylamide gel electrophoresis. Glycerol (5-10%) increases the density of a sample so that the sample will layer at the bottom of a gel’s sample well.

Glycerol is also used to aid in casting gradient gels and as a protein stabilizer and storage buffer component. Glycerol acts as a cryoprotectant and prevents formation of ice crystals and prevents cell damage. Glycerol is useful in a variety of applications, glycerol is used as a solvent, plasticizer and humectant.

In polyacrylamide gel electrophoresis, glycerol is used in sample preparation and gel formation. At a concentration of 5-10%, glycerol is used to increase the density of a sample so that it will layer at the bottom of a sample well. Additional uses include as an aid in casting gradient gels, a protein stabilizer and storage buffer component.

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.