Glycine, 0.2M buffer soln., pH 3.0
Thermo Scientific Chemicals
Glycine, 0.2M buffer soln., pH 3.0
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Quantity:
500 mL
250 mL
Catalog number J62527.AP
also known as J62527-AP
Price (USD)/ Each
105.65
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Ends: 15-Oct-2024
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Quantity:
500 mL
Request bulk or custom format
Price (USD)/ Each
105.65
Special Offer
Online exclusive
Ends: 15-Oct-2024
124.00 
Save 18.35 (15%)
Add to cart
Glycine, 0.2M buffer soln., pH 3.0
Catalog numberJ62527.AP
Price (USD)/ Each
105.65
Special Offer
Online exclusive
Ends: 15-Oct-2024
124.00 
Save 18.35 (15%)
-
Add to cart
Chemical Identifiers
CAS56-40-6
IUPAC Name2-aminoacetic acid
Molecular FormulaC2H5NO2
InChI KeyDHMQDGOQFOQNFH-UHFFFAOYSA-N
SMILESNCC(O)=O
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SpecificationsSpecification SheetSpecification Sheet
Appearance (Color)Clear colorless
FormLiquid
CommentComponent is dissolved in UV treated 18.2 megohm-cm ± 1 water, followed by pH adjustment and filtered through 1 micron filter.
pH3.0 ± 0.2
Glycine is widely used as a buffer for a variety of immunological applications. It is frequently used in the preparation of TG Buffers where the buffer is used as a running and/or transfer buffer for polyacrylamide gel electrophoresis and western blotting. Glycine is also used as an eluent in column chromatography. Glycine has been reported for use in the enzymatic determination of lactate using lactic dehydrogenase.

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

Applications
Glycine is widely used as a buffer for a variety of immunological applications. It is frequently used in the preparation of TG Buffers where the buffer is used as a running and/or transfer buffer for polyacrylamide gel electrophoresis and western blotting. Glycine is also used as an eluent in column chromatography. Glycine has been reported for use in the enzymatic determination of lactate using lactic dehydrogenase.

Solubility
Not miscible or difficult to mix in water. Slightly soluble in acetone. Insoluble in alcohol, and ether.

Notes
Store away from heat and oxidizing agents. Keep the container tightly closed and place it in a cool, dry and well ventilated condition. Keep it refrigerated.
RUO – Research Use Only

General References:

  1. H Towbin, et al. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.Proc Natl Acad Sci U S A.,1979,76(9), 4350-4354.
  2. Laemmli UK, et al. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.Nature.,1970,227(5259), 680-5.