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HEPES-buffered saline, pH 7.0 (2X for transfection)
Catalog number: J62623.AP
500 mL, Each
Thermo Scientific Chemicals
HEPES-buffered saline, pH 7.0 (2X for transfection)
Catalog number: J62623.AP
500 mL, Each
Quantity
Chemical Identifiers
CAS
7365-45-9
IUPAC Name
2-[4-(2-hydroxyethyl)piperazin-1-yl]ethane-1-sulfonic acid
Molecular Formula
C8H18N2O4S
InChI Key
JKMHFZQWWAIEOD-UHFFFAOYSA-N
SMILES
OCCN1CCN(CCS(O)(=O)=O)CC1
Specifications
Specification SheetComment
All components are dissolved in UV treated 18.2 megohm-cm ± 1 water, followed by pH adjustment and filtered through 0.22 micron filter.
Appearance (Color)
Clear colorless
Form
Liquid
pH
7.0 ± 0.2 (1X)
Description
HEPES is common buffer for biological sciences, particularly used in cell culture to maintain physiological pH. It acts a buffering component, which is used in the preparation of buffers. It is described as one of the best all-purpose buffers available for use in biological research.
This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.
General Description
- HEPES-buffered saline is an ionic buffer with a pKa=7.55
- HEPES is an ethanesulfonic acid with a 4-(2-hydroxyethyl)piperazin-1-yl substitution within one of its methyl groups
Application
- HEPES-buffered saline can be used to maintain physiological pH in a solution; it is frequently used for protein isolation and other molecular applications
- Useful for cell culture, HEPES maintains pH even in the presence of carbon dioxide
- It is intended for use in cell culture and molecular biology
RUO – Research Use Only
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