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MOPS-SDS running buffer (20X)
Catalog number: J62847.AP
500 mL, Each
Thermo Scientific Chemicals
MOPS-SDS running buffer (20X)
Catalog number: J62847.AP
500 mL, Each
Quantity
Specifications
Specification SheetAppearance (Color)
Clear pale yellow
Form
Liquid
Composition
1M MOPS, 1M Tris, 2% SDS, 20mM EDTA
Comment
All components are dissolved in UV treated 18.2 megohm-cm ± 1 water, followed by pH adjustment and filtered through 1 micron filter.
pH
7.7 ± 0.1
Description
MOPS SDS Running Buffer is recommended for separating medium- to large-sized proteins. MOPS-SDS Running Buffer is suitable for protein gel electrophoresis. Use of MOPS buffer allows proteins to run slower than when using MES buffer. This buffer is used in 1D Gel Electrophoresis, Protein Gel Electrophoresis, Proteins, Expression, Isolation and Analysis - See more at:
This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.
General Description
- MOPS-SDS running buffer is a zwitterionic, morpholinic buffer that allows the separation of medium- to large-sized proteins
Application
- MOPS is used as a running buffer in electrophoresis. The use of MOPS buffer allows proteins to run slower than when MES buffer is used
- MOPS-SDS running buffer, 20X concentrated, is formulated to be used for running proteins on an SDS gel
- This buffer contains SDS detergent to maintain the denaturation of proteins and MOPS to buffer the pH
- It is commonly used for cell culture media and protein purification in chromatography
- MOPS is frequently applied in buffered culture media for bacteria, yeast, and mammalian cells
- It is also applied in the bicinchoninic acid (BCA) assay
- It is used in separating RNA in agarose gels
RUO – Research Use Only
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