Thermo Scientific Chemicals

TRIS-buffered saline (TBS, 10X) pH 7.4, for Western blot

Catalog number: J62938.K2
1 L, Each
Thermo Scientific Chemicals

TRIS-buffered saline (TBS, 10X) pH 7.4, for Western blot

Catalog number: J62938.K2
1 L, Each
Quantity
Catalog number: J62938.K2
Price (USD)
Price: 65.50
Online price: 55.65
Your price:
Quantity
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Chemical Identifiers

CAS
77-86-1
IUPAC Name
2-amino-2-(hydroxymethyl)propane-1,3-diol
Molecular Formula
C4H11NO3
InChI Key
LENZDBCJOHFCAS-UHFFFAOYSA-N
SMILES
NC(CO)(CO)CO
Appearance (Color)
Clear colorless
Comment
All components are dissolved in UV treated 18.2 megohm-cm ñ 1 water, followed by pH adjustment and 1 micron filtration
Form
Liquid
pH
7.4 ñ 0.2 (1X concentration)

Description

For Western blot washing.TRIS-buffered saline (TBS, 10X) is used as a washing buffer for electrodes, alkaline phosphatase and peroxidase conjugates in dot blot assay. Further, it acts as a pH stabilizer, which enable washing without disruption of antibody-antigen binding interactions.

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

Applications
For Western blot washing.TRIS-buffered saline (TBS, 10X) is used as a washing buffer for electrodes, alkaline phosphatase and peroxidase conjugates in dot blot assay. Further, it acts as a pH stabilizer, which enable washing without disruption of antibody-antigen binding interactions.

Solubility
Miscible with water.

Notes
500mM Tris, 1.5M NaCl, pH 7.4.Store in a cool place. Incompatible with strong oxidizing agents.
RUO – Research Use Only

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