The primary purpose of the PMT ELISA is to demonstrate toxigenic strains of P. multocida in bacterial specimens obtained from pigs, since this is the essential criterion for the laboratory diagnosis of progressive atrophic rhinitis.1, 2 However, some laboratory situations require a method for determination of antibodies to PMT.3 This is especially the case in animal health surveillance systems based on screening of serum or colostrum specimens for antibodies to PMT. Furthermore, serological analysis is important when monitoring efficacy of vaccines for progressive atrophic rhinitis by determination of anti-PMT titres.
Use Thermo Scientific Oxoid Accessory Reagents for PMT Serology in conjunction with Thermo Scientific Oxoid Pasteurella multocida Toxin PMT Kit to detect antibodies to PMT from serum and colostrum specimens.
This procedure is independent of the animal source of the antibodies and, therefore, can be used for detection of anti-PMT antibodies in serum or colostrum from any animal species.
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1. Foged NT, Lou H, Mattsson S. A 1-step ELISA for the diagnosis of progressive atrophic rhinitis. Proceedings World Association of Veterinary Laboratory Diagnosticians Congress 1992:21.
2. Mattsson S, Foged NT, Soderlind O. Evaluation of the 1-step DAKO PMT ELISA for detection of toxin producing Pasteurella multocida. Proceedings International Pig Veterinary Society Congress 1992:173.
3. Foged NT. Pasteurella multocida toxin. The characterisation of the toxin and its significance in the diagnosis and prevention of progressive atrophic rhinitis in pigs. APMIS 1992;100 Suppl 25:1-56.