Thermo Scientific™

PCR Master Mix (2X)

Catalog number:  K0171

Related applications:

PCR

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Description

Thermo Scientific PCR Master Mix is a 2X concentrated solution of Taq DNA Polymerase, dNTPs, and all of the components required for PCR, except DNA template and primers. This pre-mixed formulation saves time and reduces contamination due to a reduced number of pipetting steps required for PCR set up. The mix is optimized for efficient and reproducible PCR.

Highlights

Convenient, ready-to-use mix
Thermostable—half life is more than 40 min at 95°C
Generates PCR products with 3'-dA overhangs
Incorporates modified nucleotides (e.g., biotin-, digoxigenin-, fluorescently-labeled nucleotides)

Applications

• Routine PCR amplification of DNA fragments up to 5 kb
• High throughput PCR
• DNA labeling

Note

• The error rate of Taq DNA Polymerase in PCR is 2.2 x 10-5 errors per nt per cycle, as determined by a modified method described in. Accordingly, the accuracy of PCR is 4.5 x 104. Accuracy is an inverse of the error rate and shows an average number of correct nucleotides incorporated before an error occurs.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Fidelity (vs. Taq): 1 X
GC-Rich PCR Performance: Low
Hot Start: No
Polymerase: Taq DNA Polymerase
Product Overhang: 3'-A
Product Size: 200 reactions
Reaction Format: SuperMix or Master Mix
Reaction Speed: Standard

Contents & storage

Contains:
• 4 x 1.25 mL PCR Master Mix (2X), which includes:
— Taq DNA polymerase (0.05 U/µL),
— reaction buffer,
— 4 mM MgCl2, and
— 0.4 mM of each dNTP
• 4 x 1.25 mL Nuclease-free water

Store at -20°C.