Thermo Scientific™

Maxima SYBR Green qPCR Master Mix (2X), with separate ROX vial

Catalog number: K0252
Thermo Scientific™

Maxima SYBR Green qPCR Master Mix (2X), with separate ROX vial

Catalog number: K0252
Catalog Number
K0252
Unit Size
1,000 reactions
Price (USD)
810.00
Your Price:
Availability
***
Quantity
Catalog Number
Unit Size
200 reactions
Price (USD)
191.00
Your Price:
Availability
***
Quantity
Catalog Number
Unit Size
4,000 reactions
Price (USD)
2,270.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
K02521,000 reactions
810.00
Your Price:
***
K0251200 reactions
191.00
Your Price:
***
K02534,000 reactions
2,270.00
Your Price:
***
Product Overview
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Alternative Product: Try PowerUp SYBR Green Master Mix, our newest, high-performance, SYBR dye-based master mix for superior performance at a very competitive price. With PowerUp SYBR Green Master Mix, we’ve taken the best of Maxima SYBR Green qPCR Master Mix and added additional capabilities for your gene expression analysis.

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Thermo Scientific Maxima SYBR Green qPCR Master Mix is a ready-to-use solution optimized for qPCR and 2-step RT-qPCR. The master mixes include Maxima Hot Start Taq DNA Polymerase and dNTPs in an optimized PCR buffer. Only template and primers need to be added. The SYBR Green I intercalating dye allows for DNA detection and analysis without using sequence-specific probes.

Maxima Hot Start Taq DNA Polymerase in combination with an optimized buffer ensures PCR specificity and sensitivity. dUTP is included in the mix for optional carry-over contamination control using uracil DNA glycosylase (UDG) (1). The use of Maxima SYBR Green qPCR Master Mixes in real-time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral, and cDNA templates. Maxima SYBR Green qPCR Master Mixes are compatible with most most real-time thermal cyclers (see Resources for a compatibility chart).

Highlights

SpecificityMaxima Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers
Sensitivity—detects low copy number targets
Wide linear range—accurate quantification across 9 orders of magnitude
Reproducibility and convenience—ready-to-use 2x master mix

Applications

• Gene expression
• siRNA validation
• Genotyping
• Pathogen detection

Includes

Maxima SYBR Green qPCR Master Mix, ROX Solution provided (2x) includes Maxima Hot Start Taq DNA Polymerase and dNTPs (also dUTP) in an optimized PCR buffer. Supplied with nuclease-free water and a separate ROX vial.

Related Products
Maxima SYBR Green/ROX qPCR Master Mix (2X)
Maxima SYBR Green/Fluorescein qPCR Master Mix (2X)
Maxima SYBR Green qPCR Master Mix (2X), with separate ROX vial
For Research Use Only. Not for use in diagnostic procedures.

Specifications

For Use With (Application)
Real Time PCR (qPCR)
For Use With (Equipment)
BioRad CFX96™, MJ Chromo4, MJ Opticon, Eppendorf MasterCycler RealPlex, Bio-Rad CFX384 Touch™, Roche LightCycler 480, Cepheid SmartCycler, Corbett RotorGene
Product Line
Maxima
Product Type
Real Time PCR SYBR Master Mix
Sufficient For
1000 Reactions at 25 μL
Concentration
2X
Detection Method
SYBR
Format
Tube
GC-Rich PCR Performance
High
No. of Reactions
1,000 Reactions
PCR Method
qPCR
Polymerase
Taq DNA Polymerase
Reaction Speed
Standard
Sample Type
DNA, dsDNA
Volume
10 x 1.25 mL

Contents & Storage

Contains:
• 10 x 1.25 mL Maxima SYBR Green qPCR Master Mix (2X) containing Maxima Hot Start Taq DNA Polymerase, dNTPs (also dUTP) and SYBR Green I in an optimized PCR buffer; sufficient for 1000 x 25 µL reactions.
• 250 µL ROX solution, 50 µM
• 10 x 1.25 mL Nuclease-Free Water

Store at -20°C.

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Frequently asked questions (FAQs)

Citations & References