Thermo Scientific™

Luminaris Color Probe qPCR Master Mix

Catalog number: K0351
Thermo Scientific™

Luminaris Color Probe qPCR Master Mix

Catalog number: K0351
Catalog Number
K0351
Unit Size
250 reactions
Price (USD)
246.00
Your Price:
Availability
***
Quantity
Catalog Number
Unit Size
1250 reactions
Price (USD)
1,014.00
Your Price:
Availability
***
Quantity
Catalog Number
Unit Size
5,000 reactions
Price (USD)
2,920.00
Your Price:
Availability
***
Quantity
Catalog Number
Unit Size
500 reactions
Price (USD)
447.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
K0351250 reactions
246.00
Your Price:
***
K03531250 reactions
1,014.00
Your Price:
***
K03545,000 reactions
2,920.00
Your Price:
***
K0352500 reactions
447.00
Your Price:
***
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Recommendations
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Alternative Product: Try TaqMan Fast Advanced Master Mix, our highest-performance, probe-based master mix. With TaqMan Fast Advanced Master Mix, we’ve taken the best of Luminaris Color Probe qPCR Master Mix and added additional capabilities for your gene expression analysis.

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Thermo Scientific Luminaris Color Probe and Luminaris Probe qPCR Master Mixes are universal ready-to-use solutions optimized for qPCR and two-step RT-qPCR. The master mixes include Thermo Scientific Hot Start Taq DNA polymerase, uracil-DNA glycosylase (UDG) and dNTPs in an optimized PCR buffer. Only the template, primers and probe need to be added.

The Hot Start Taq DNA polymerase, in combination with an optimized buffer, ensures PCR specificity and sensitivity. dUTP and UDG are included in the mix for carry-over contamination control (see Reference 1). The Luminaris Color Probe qPCR Master Mixes are supplemented with an inert blue dye and a separate Yellow Sample Buffer that contains a yellow dye. The qPCR reaction mix containing both components is green.

The use of Luminaris Color Probe and Luminaris Probe qPCR Master Mixes in qPCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates. Individual formulations of Luminaris Color Probe and Luminaris Probe qPCR Master Mixes with ROX at different concentrations as passive reference dye are available for most qPCR platforms (see Real-time PCR instrument compatibility chart).

Highlights

• Blue Master Mix and Yellow Sample Buffer for easy pipetting
• UDG in the master mix to prevent carry-over contamination• Specificity—Hot Start Taq DNA Polymerase and the optimized buffer reduce primer dimers
• Sensitivitity—single copy detection
• Wide linear range—accurate quantification across 9 orders of magnitude
• Reproducibility and convenience— ready-to-use 2X master mix
• Equal performance of blue and colorless master mixes
• Versions with premixed ROX for different real-time platforms
• Standard cycling protocols

Applications

• Gene expression analysis
• siRNA validation
• Genotyping
• Pathogen detection

Includes

Luminaris Color Probe qPCR Master Mix (2X) includes Hot Start Taq Polymerase, UDG and dNTPs (also dUTP) in an optimized PCR buffer with a blue dye; supplied with 40X Yellow Sample Buffer and nuclease-free water.

Specifications

Description
Color Probe
Concentration
2X (Luminaris Color Probe qPCR Master Mix), 40X (Yellow Sample Buffer)
Detection Method
Primer-Probe Detection
Format
Tube
GC-Rich PCR Performance
High
No. of Reactions
250 Reactions
PCR Method
qPCR
Polymerase
Taq DNA Polymerase
Reaction Speed
Standard
Sample Type
DNA, dsDNA
Volume
2 x 1.25 mL
For Use With (Application)
Real Time PCR (qPCR)
For Use With (Equipment)
BioRad CFX96™, BioRad iCycler iQ, BioRad iQ5, BioRad MyiQ, MJ Chromo4, Eppendorf MasterCycler RealPlex, Corbett RotorGene, Bio-Rad CFX384 Touch™, Roche LightCycler 480, Cepheid SmartCycler, MJ Opticon
Product Line
Luminaris
Product Type
qPCR Master Mix
Quantity
250 x 20µL rxns
Sufficient For
250 Reactions of 20 μL

Contents & Storage

Contains:
• 2 x 1.25 mL Luminaris Color Probe qPCR Master Mix (2X) containing Hot Start Taq DNA Polymerase, UDG and dNTPs (with dUTP) in an optimized PCR buffer with a blue dye; sufficient for 250 x 20 µL reactions
• 1 x 1.25 mL 40X Yellow Sample Buffer
• 2 x 1.25 mL Nuclease-Free Water

Store at -20°C.

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