Thermo Scientific™

Luminaris Color HiGreen qPCR Master Mix, low ROX

Catalog number: K0371
Thermo Scientific™

Luminaris Color HiGreen qPCR Master Mix, low ROX

Catalog number: K0371
Catalog Number
K0371
Unit Size
250 reactions
Price (USD)
239.00
Your Price:
Availability
***
Quantity
Catalog Number
Unit Size
5,000 reactions
Price (USD)
2,820.00
Your Price:
Availability
***
Quantity
Catalog Number
Unit Size
1250 reactions
Price (USD)
1,002.00
Your Price:
Availability
***
Quantity
Catalog Number
Unit Size
500 reactions
Price (USD)
435.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
K0371250 reactions
239.00
Your Price:
***
K03745,000 reactions
2,820.00
Your Price:
***
K03731250 reactions
1,002.00
Your Price:
***
K0372500 reactions
435.00
Your Price:
***
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Recommendations
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Alternative Product: Try PowerUp SYBR Green Master Mix, our newest, high-performance, SYBR dye-based master mix for superior performance at a very competitive price. With PowerUp SYBR Green Master Mix, we’ve taken the best of Luminaris Color HiGreen qPCR Master Mix and added additional capabilities for your gene expression analysis.

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Thermo Scientific Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes are universal ready-to-use solutions optimized for qPCR and two-step RT-qPCR. The master mixes include Thermo Scientific Hot Start Taq DNA polymerase, uracil-DNA glycosylase (UDG) and dNTPs in an optimized PCR buffer. Only the template and primers need to be added. The SYBR Green I intercalating dye allows for DNA detection and analysis without using sequence-specific probes.

The Hot Start Taq DNA polymerase in combination with an optimized buffer ensures PCR specificity and sensitivity. dUTP and UDG are included in the mix for carry-over contamination control (see Reference 1). The Luminaris Color HiGreen qPCR Master Mixes are supplemented with an inert blue dye and a separate Yellow Sample Buffer that contains a yellow dye. The qPCR reaction mix containing both components is green.

The use of Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes in qPCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates. Individual formulations of Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes with ROX at different concentrations or fluorescein as passive reference dyes are available for most qPCR platforms (see Real-time PCR instrument compatibility chart).

Highlights

• Blue master mix and Yellow Sample Buffer for easy pipetting
• UDG in the master mix to prevent carry-over contamination
• Equal performance of blue and colorless master mixes
• Versions with premixed ROX or fluorescein for different real-time platforms
• Standard cycling protocols
• Excellent qPCR performance:
Specificity: Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers
Sensitivity: single copy detection
Wide linear range: accurate quantification across 9 orders of magnitude
Reproducibility and convenience: ready-to-use 2X master mix


Applications

• Gene expression analysis
• siRNA validation
• Genotyping
• Pathogen detection

Includes

Luminaris Color HiGreen Low ROX qPCR Master Mix (2X) includes Hot Start Taq DNA Polymerase, UDG and dNTPs (also dUTP) in an optimized PCR buffer with a blue dye. The master mix contains low concentration of ROX passive reference dye for specific real-time platforms (see Real-Time PCR instrument compatibility chart) and is supplied with 40X Yellow Sample Buffer and nuclease-free water.

Specifications

Concentration
2 X
Detection Method
SYBR
Fidelity (vs. Taq)
2 X
GC-Rich PCR Performance
High
No. of Reactions
250 Reactions
PCR Method
qPCR
Polymerase
Taq DNA Polymerase
Reaction Speed
Standard
Sample Type
DNA, dsDNA
For Use With (Application)
qPCR, Two-Step RT-qPCR
For Use With (Equipment)
7500 System, QuantStudio™, ViiA™ 7 System, Stratagene Mx4000, Stratagene Mx3000P, Stratagene Mx3005P
No. of Tests
250 Reactions
Product Line
Luminaris
Product Type
qPCR Master Mix
Sufficient For
250 Reactions

Contents & Storage

Contains:
• 2 x 1.25 mL Luminaris Color HiGreen Low ROX qPCR Master Mix (2X) containing Hot Start Taq DNA Polymerase, UDG, dNTPs (with dUTP) and SYBR Green I in an optimized PCR buffer with a blue dye; sufficient for 250 x 20 µL reactions. The master mix contains low concentration of ROX passive reference dye for specific real-time platforms.
• 1 x 1.25 mL 40X Yellow Sample Buffer
• 2 x 1.25 mL Nuclease-Free Water

Store at -20°C.

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