Thermo Scientific™

GeneJET Plasmid Midiprep Kit

Catalog number: K0482
Thermo Scientific™

GeneJET Plasmid Midiprep Kit

Catalog number: K0482
Catalog Number
K0482
Unit Size
100 preps
Price (USD)
Price: 816.00
Your Price:
Availability
***
Quantity
Catalog Number
Unit Size
25 preps
Price (USD)
Price: 225.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
K0482100 preps
Price: 816.00
Your Price:
***
K048125 preps
Price: 225.00
Your Price:
***
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Thermo Scientific GeneJET Plasmid Midiprep Kit, sufficient for 100 preps, is designed for large scale isolation of high quality plasmid DNA from recombinant E. coli cultures. The kit utilizes silica-based membrane technology in the form of convenient spin column. Each prep recovers up to 200 µg of high copy number plasmid DNA. The purified plasmid DNA can be used in a wide variety of molecular biology procedures, such as restriction endonuclease digestion, PCR, cloning, transformation, automated sequencing, in vitro transcription, and transfection of robust cell lines.

Highlights

Efficient—high yields up to 200 µg of high quality plasmid DNA from 50 mL of bacterial culture
High concentration of DNA purified—> 400 ng/ µL
Convenient—spin column format, centrifuge or vacuum protocols
Fast—procedure takes 60 minutes, considerably faster than using gravity flow columns

Applications

• Isolated plasmid DNA is suitable for all conventional molecular biology procedures, including:
FastDigest restriction enzyme digestion
• Automated fluorescent sequencing
• PCR
• Cloning
• Transformation
In vitro transcription
• Transfection of robust cell lines
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Concentration
400 ng/µL
Column Type
Spin Column
Format
Spin Column
Isolation Technology
Silica Column
Sample Type
Bacteria
Scale
Midi
For Use With (Application)
Real-Time Quantitative PCR (qPCR), Transfection, Cloning, Sequencing, In Vitro Transcription, Transformation, Nucleic Acid Labeling, PCR
High-throughput Compatibility
Not High-throughput Compatible (Manual)
Prep Scale
100-200 µg (Medium-Scale) Plasmid DNA
Product Line
GeneJET
Product Type
Plasmid MidiPrep Kit
Quantity
100 Preps
Target
Plasmid DNA
Test Time
60 min.

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