Thermo Scientific™

GeneJET Plasmid Maxiprep Kit

Catalog number:  K0491

Related applications:

DNA & RNA Purification & Analysis

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Description

Thermo Scientific GeneJET Plasmid Maxiprep Kit, sufficient for 25 preps, utilizes silica-based membrane technology in the form of convenient spin column. Each prep recovers up to 750 µg of high copy plasmid DNA that can be used in a wide variety of molecular biology procedures, such as restriction endonuclease digestion, PCR, cloning, transformation, automated sequencing, in vitro transcription, and transfection of robust cell lines.

Highlights

Efficient—high yields up to 750 µg of high quality plasmid DNA from 250 mL of bacterial culture
High concentration of DNA purified—> 400 ng/ µL
Convenient—spin column format, centrifuge or vacuum protocols
Fast—procedure takes 60 minutes, considerably faster than using gravity flow columns

Applications

• Isolated plasmid DNA is suitable for all conventional molecular biology procedures, including:
• Transfection of robust cell lines
FastDigest restriction enzyme digestion
• Automated fluorescent sequencing
• PCR
• Cloning
• Transformation
In vitro transcription
For Research Use Only. Not for use in diagnostic procedures.

Specifications

For Use With (Application): Real-Time Quantitative PCR (qPCR), Transfection, Cloning, Sequencing, In Vitro Transcription, Transformation, Nucleic Acid Labeling, PCR
Purification Target: Plasmid DNA
Sample Type: Bacteria
No. of Reactions: 10 Preps
High-throughput Compatibility: Not High-throughput Compatible (Manual)
Prep Scale: > 200 µg (Large-Scale) Plasmid DNA
Product Line: GeneJET