Thermo Scientific™

GeneJET Plasmid Miniprep Kit

Catalog number: K0502
Thermo Scientific™

GeneJET Plasmid Miniprep Kit

Catalog number: K0502
Catalog Number
K0502
Unit Size
50 preps
Price (USD)
Price: 78.00
Your Price:
Availability
-
Quantity
Catalog Number
Unit Size
250 preps
Price (USD)
Price: 321.00
Your Price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
K050250 preps
Price: 78.00
Your Price:
-
K0503250 preps
Price: 321.00
Your Price:
-
Product Overview
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Thermo Scientific GeneJET Plasmid Miniprep Kit utilizes an exclusive silica-based membrane technology in the form of a convenient spin column. The kit recovers up to 20 µg of high copy plasmid DNA per isolation procedure.

Highlights

Efficient—high yields of up to 20 µg of high quality plasmid DNA
Fast—procedure takes less than 14 minutes
Convenient—no phenol/chloroform extraction or alcohol precipitation required
Pure—purified DNA is immediately ready to use

Applications

• Fast isolation of high purity plasmid DNA suitable for all conventional molecular biology procedures, including:
• FastDigest or conventional restriction digestion
• Automated fluorescent and radioactive sequencing
• PCR
In vitro transcription
• Transformation

Principle

A bacterial culture is harvested and lysed. The lysate is then cleared by centrifugation and applied on the silica column to selectively bind DNA molecules at a high salt concentration. The adsorbed DNA is washed to remove contaminants, and the pure plasmid DNA is eluted in a small volume of elution buffer or water. The purified DNA is ready for immediate use in all molecular biology procedures, such as fast and conventional digestion with restriction enzymes, PCR, in vitro transcription, transformation, and automated sequencing. Components Resuspension Solution Lysis Solution Neutralization Solution Wash Solution (concentrated) RNase A Solution Elution Buffer Spin Columns Collection Tubes Detailed Protocol

Related Products
RNase A
Elution Buffer for GeneJET Plasmid Miniprep Kit
Lysis Solution for GeneJET Plasmid Miniprep Kit
Resuspension Solution for GeneJET Plasmid Miniprep Kit
Neutralization Solution for GeneJET Plasmid Miniprep Kit
Wash Solution for GeneJET Plasmid Miniprep Kit
Binding Buffer for GeneJET Plasmid Miniprep Kit
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Column Type
Spin Column
Format
Spin Column
Isolation Technology
Silica Column
Sample Type
Bacteria
Scale
Mini
For Use With (Application)
PCR, Cloning, Sequencing, Transformation, Nucleic Acid Labeling, In Vitro Transcription
High-throughput Compatibility
Compatible (Vacuum manifold)
No. of Reactions
50 Preps
Prep Scale
< 100 µg (Small-Scale) Plasmid DNA
Product Line
GeneJET
Product Type
Plasmid MiniPrep Kit
Quantity
50 Preps
Target
Plasmid DNA
Test Time
<14 min