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Thermo Scientific™
Genomic DNA Purification Kit
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Description
Thermo Scientific Genomic DNA Purification Kit is a simple and rapid system for high quality genomic DNA purification from various sources including: whole blood, serum, cell lines, bacterial cells, plant, and mammalian tissues.The kit is based on selective detergent-mediated DNA precipitation from crude lysate. The entire procedure is rapid only 20 to 25 minutes with a typical yield of 2 to 10 µg genomic DNA from 0.2 mL of blood.
High molecular weight genomic DNA purified with the kit is suitable for direct use in all common molecular biology applications: PCR, FastDigest and conventional restriction digestion, cloning, DNA sequencing, and Southern blot analysis.
Highlights
• Efficient—2 to 10 µg of genomic DNA from 0.2 mL of blood
• Fast—procedure takes approximately 20 minutes
• Universal—purifies genomic DNA from various sources
• Flexible—easy to scale up and down
• Safe—does not involve phenol extraction
Applications
• Purification of high-quality genomic DNA from:
• Whole blood or serum
• Cell culture
• Plant tissues
• Mammalian tissues
• Epithelium samples
• Bacteria Includes Lysis Solution Precipitation Solution NaCl Solution Detailed Protocol
For Research Use Only. Not for use in diagnostic procedures.
Specifications
| Sample Type (General): | Bacteria, Blood, Cells, Liquid Samples (e.g. Serum), Plant Samples, Tissue, Yeast |
|---|---|
| Downstream Application: | Cloning, PCR, Real-Time Quantitative PCR (qPCR), Sequencing, Southern Blotting |
| High Throughput Compatibility: | Not High Throughput-Compatible (Manual) |
| Product Size: | 100 preps |
Contents & storage
• Lysis Solution, 40 mL
• Precipitation Solution (10X), 8 mL
• 1.2 M sodium chloride, 10 mL
Store at room temperature.
• Precipitation Solution (10X), 8 mL
• 1.2 M sodium chloride, 10 mL
Store at room temperature.
