Genomic DNA Purification Kit

      Catalog number:  K0512

      Thermo Scientific™  Related applications: DNA Extraction

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      Description

      Thermo Scientific Genomic DNA Purification Kit is a simple and rapid system for high quality genomic DNA purification from various sources including: whole blood, serum, cell lines, bacterial cells, plant, and mammalian tissues.

      The kit is based on selective detergent-mediated DNA precipitation from crude lysate. The entire procedure is rapid only 20 to 25 minutes with a typical yield of 2 to 10 µg genomic DNA from 0.2 mL of blood.

      High molecular weight genomic DNA purified with the kit is suitable for direct use in all common molecular biology applications: PCR, FastDigest and conventional restriction digestion, cloning, DNA sequencing, and Southern blot analysis.

      Highlights

      • Efficient—2 to 10 µg of genomic DNA from 0.2 mL of blood
      • Fast—procedure takes approximately 20 minutes
      • Universal—purifies genomic DNA from various sources
      • Flexible—easy to scale up and down
      • Safe—does not involve phenol extraction

      Applications

      • Purification of high-quality genomic DNA from:
      • Whole blood or serum
      • Cell culture
      • Plant tissues
      • Mammalian tissues
      • Epithelium samples
      • Bacteria Includes Lysis Solution Precipitation Solution NaCl Solution Detailed Protocol
      For Research Use Only. Not for use in diagnostic procedures.

      Specifications

      Sample Type (General): Bacteria, Blood, Cells, Liquid Samples (e.g. Serum), Plant Samples, Tissue, Yeast
      Downstream Application: Cloning, PCR, Real-Time Quantitative PCR (qPCR), Sequencing, Southern Blotting
      High Throughput Compatibility: Not High Throughput-Compatible (Manual)
      Product Size: 100 preps

      Contents & storage

      • Lysis Solution, 40 mL
      • Precipitation Solution (10X), 8 mL
      • 1.2 M sodium chloride, 10 mL

      Store at room temperature.

      Documents

      Manuals & protocols