Thermo Scientific™

Genomic DNA Purification Kit

Catalog number:  K0512

Related applications:

DNA Extraction

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Description

Thermo Scientific Genomic DNA Purification Kit is a simple and rapid system for high quality genomic DNA purification from various sources including: whole blood, serum, cell lines, bacterial cells, plant, and mammalian tissues.

The kit is based on selective detergent-mediated DNA precipitation from crude lysate. The entire procedure is rapid only 20 to 25 minutes with a typical yield of 2 to 10 µg genomic DNA from 0.2 mL of blood.

High molecular weight genomic DNA purified with the kit is suitable for direct use in all common molecular biology applications: PCR, FastDigest and conventional restriction digestion, cloning, DNA sequencing, and Southern blot analysis.

Highlights

Efficient—2 to 10 µg of genomic DNA from 0.2 mL of blood
Fast—procedure takes approximately 20 minutes
Universal—purifies genomic DNA from various sources
Flexible—easy to scale up and down
Safe—does not involve phenol extraction

Applications

• Purification of high-quality genomic DNA from:
• Whole blood or serum
• Cell culture
• Plant tissues
• Mammalian tissues
• Epithelium samples
• Bacteria Includes Lysis Solution Precipitation Solution NaCl Solution Detailed Protocol
For Research Use Only. Not for use in diagnostic procedures.

Specifications

For Use With (Application): Real-Time Quantitative PCR (qPCR), Cloning, Southern Blotting, Sequencing, PCR
Purification Target: Genomic DNA
Sample Type: Liquid Samples (e.g. Serum), Plant Samples, Cells, Tissue, Yeast, Bacteria, Blood
High-throughput Compatibility: Not High-throughput Compatible (Manual)

Contents & storage

• Lysis Solution, 40 mL
• Precipitation Solution (10X), 8 mL
• 1.2 M sodium chloride, 10 mL

Store at room temperature.