Thermo Scientific™

Genomic DNA Purification Kit

Número do catálogo: K0512
Thermo Scientific™

Genomic DNA Purification Kit

Número do catálogo: K0512
Número do catálogo
K0512
Tamanho da unidade
100 preps
Número do catálogoTamanho da unidadePreço (BRL)
K0512100 prepsEntre em contato ›
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Thermo Scientific Genomic DNA Purification Kit is a simple and rapid system for high quality genomic DNA purification from various sources including: whole blood, serum, cell lines, bacterial cells, plant, and mammalian tissues.

The kit is based on selective detergent-mediated DNA precipitation from crude lysate. The entire procedure is rapid only 20 to 25 minutes with a typical yield of 2 to 10 μg genomic DNA from 0.2 mL of blood.

High molecular weight genomic DNA purified with the kit is suitable for direct use in all common molecular biology applications: PCR, FastDigest and conventional restriction digestion, cloning, DNA sequencing, and Southern blot analysis.

Highlights

Efficient—2 to 10 μg of genomic DNA from 0.2 mL of blood
Fast—procedure takes approximately 20 minutes
Universal—purifies genomic DNA from various sources
Flexible—easy to scale up and down
Safe—does not involve phenol extraction

Applications

• Purification of high-quality genomic DNA from:
• Whole blood or serum
• Cell culture
• Plant tissues
• Mammalian tissues
• Epithelium samples
• Bacteria Includes Lysis Solution Precipitation Solution NaCl Solution Detailed Protocol

Especificações

Isolation Technology
Organic Extraction
Sample Type
Bacteria, Cells, Plant, Serum, Tissue, Whole Blood, Blood
Elution Volume
20 to 100 μL
Final Product Type
gDNA
For Use With (Application)
PCR, Southern blotting, sequencing, nucleic acid labeling, hybridization
High-throughput Compatibility
Not High-throughput Compatible (Manual)
Quantity
100 Preps
Shipping Condition
Room Temperature
Test Time
25 min.
Starting Material Amount
Bacteria: ≤20 mg
Cells: ≤106
Plant: ≤100 mg
Tissue: ≤30 mg
Serum: ≤200 μL
Whole Blood: ≤200 μL

Conteúdo e armazenamento

• 40 mL Lysis Solution; room temperature
• 8 mL Precipitation Solution (concentrated); room temperature
• 10 mL NaCl Solution; room temperature

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