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GeneJET Whole Blood RNA Purification Mini KitThermo Scientific GeneJET Whole Blood RNA Purification Mini Kit is designed for rapid and efficient purification of high quality totalRead more
Catalog number K0761
Price (USD)/ 50 preps
268.00
-
Price (USD)/ 50 preps
268.00
GeneJET Whole Blood RNA Purification Mini Kit
Catalog numberK0761
Price (USD)/ 50 preps
268.00
-
Thermo Scientific GeneJET Whole Blood RNA Purification Mini Kit is designed for rapid and efficient purification of high quality total RNA from whole blood and related body fluids.
The kit utilizes silica-based membrane technology in the form of a convenient spin column, eliminating the need for expensive resins, toxic phenol-chloroform extractions, or time-consuming alcohol precipitation. The standard procedure takes less than 15 minutes. The purified high quality RNA can be used in a wide range of downstream applications, such as RT-PCR, RT-qPCR, Northern blotting, and other RNA-based analysis.
Highlights
• Efficient – up to 1.8 μg of total RNA from 500 μL of human blood
• Convenient – spin columns are capped and assembled with collection tubes
• Pure – RNA pure from enzyme inhibitors, proteins, ready to use in all demanding applications. Validated in cDNA synthesis and quantitative RT-PCR
• Fast and simple – procedure takes less than 15 minutes and does not involve organic extraction or differential RBC lysis
• Compatible – suitable for vide variety of samples - human and animal blood treated with EDTA, citrate or heparin, buffy coat, bone marrow, and related body fluids
Applications
• Fast extraction of high purity RNA suitable for all conventional molecular biology procedures, including:
• RT-PCR
• RT-qPCR
• Northern blotting
• Nuclease protection assaysncludes Lysis Buffer Wash Buffer WB 1 (concentrated) Wash Buffer 2 (concentrated) Water, nuclease-free GeneJET RNA Purification Columns (pre-assembled with collection tubes) Collection tubes, 2 mL Collection tubes, 1.5 mL Detailed Protocol
The kit utilizes silica-based membrane technology in the form of a convenient spin column, eliminating the need for expensive resins, toxic phenol-chloroform extractions, or time-consuming alcohol precipitation. The standard procedure takes less than 15 minutes. The purified high quality RNA can be used in a wide range of downstream applications, such as RT-PCR, RT-qPCR, Northern blotting, and other RNA-based analysis.
Highlights
• Efficient – up to 1.8 μg of total RNA from 500 μL of human blood
• Convenient – spin columns are capped and assembled with collection tubes
• Pure – RNA pure from enzyme inhibitors, proteins, ready to use in all demanding applications. Validated in cDNA synthesis and quantitative RT-PCR
• Fast and simple – procedure takes less than 15 minutes and does not involve organic extraction or differential RBC lysis
• Compatible – suitable for vide variety of samples - human and animal blood treated with EDTA, citrate or heparin, buffy coat, bone marrow, and related body fluids
Applications
• Fast extraction of high purity RNA suitable for all conventional molecular biology procedures, including:
• RT-PCR
• RT-qPCR
• Northern blotting
• Nuclease protection assaysncludes Lysis Buffer Wash Buffer WB 1 (concentrated) Wash Buffer 2 (concentrated) Water, nuclease-free GeneJET RNA Purification Columns (pre-assembled with collection tubes) Collection tubes, 2 mL Collection tubes, 1.5 mL Detailed Protocol
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Isolation TechnologySilica Spin Column
Purification Time15 min. (After sample homogenization)
Elution Volume50 μL
Sample TypeBlood, Bone Marrow, Buffy Coat
Final Product TypeTotal RNA
High-throughput CompatibilityNot High-throughput Compatible (Manual)
For Use With (Application)RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
Quantity50 Preps
ScaleMini
Shipping ConditionRoom Temperature
Starting Material AmountBlood: 50–500 μL
Bone Marrow: 50–500 μL
Buffy Coat: 500 μL
Bone Marrow: 50–500 μL
Buffy Coat: 500 μL
YieldUp to 11 μg
Unit Size50 preps
Contents & Storage
• 40 mL Lysis Buffer; store at room temperature
• 40 mL Wash Buffer WB1 (concentrated); room temperature
• 23 mL Wash Buffer 2 (concentrated); room temperature
• 30 mL Nuclease-free Water; room temperature
• 50 GeneJET RNA Spin Columns with Collection Tubes; room temperature
• 50 Collection Tubes (2 mL); room temperature
• 50 Collection Tubes (1.5 mL); room temperature
• 40 mL Wash Buffer WB1 (concentrated); room temperature
• 23 mL Wash Buffer 2 (concentrated); room temperature
• 30 mL Nuclease-free Water; room temperature
• 50 GeneJET RNA Spin Columns with Collection Tubes; room temperature
• 50 Collection Tubes (2 mL); room temperature
• 50 Collection Tubes (1.5 mL); room temperature