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Thermo Scientific™

GeneJET Whole Blood Genomic DNA Purification Mini Kit

Catalog number: K0782
Thermo Scientific™

GeneJET Whole Blood Genomic DNA Purification Mini Kit

Catalog number: K0782
Catalog Number
K0782
Unit Size
250 preps
Price (USD)
Price: 702.00
Your price:
Availability
-
Quantity
Catalog Number
Unit Size
50 preps
Price (USD)
Price: 157.00
Your price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
K0782250 preps
Price: 702.00
Your price:
-
K078150 preps
Price: 157.00
Your price:
-
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Thermo Scientific GeneJET Whole Blood Genomic DNA Purification Mini Kit utilizes silica-based membrane technology in the form of a convenient spin column, eliminating the need for expensive resins, toxic phenol-chloroform extractions, or time-consuming alcohol precipitation. The standard procedure takes less than 20 minutes following cell lysis and yields purified DNA greater than 30 kb in size. Isolated DNA can be used directly in PCR, Southern blotting, and enzymatic reactions.

Highlights
Efficient—high yields of high molecular weight genomic DNA
Fast—20 minute procedure following cell lysis
Convenient—spin columns are capped and assembled with collection tubes
Pure—DNA pure from enzyme inhibitors, RNA and proteins. Ready to use for all demanding applications and validated in real-time PCR, endpoint PCR, multiplex PCR, and restriction enzyme digests
Compatible—suitable for wide variety of samples like fresh or frozen anticoagulated mammalian blood (treated with EDTA, Citrate or Heparine), aged blood, buffy coat, bone marrow, and body fluids

Applications
• Isolated genomic DNA is ideal for use in common molecular biology procedures, including:
• PCR and qPCR
• FastDigest and conventional restriction digestion
• Southern blotting Includes Proteinase K Solution Lysis Solution Wash Buffer WB I (concentrated) Wash Buffer II (concentrated) Elution Buffer GeneJET Purification Columns (pre-assembled with collection tubes) Collection tubes Detailed Protocol

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Column Type
Spin Column
Isolation Technology
Silica Spin Column
Sample Type
Bone Marrow, Buffy Coat, Dried Blood Spots, Buccal Swab, Urine, Whole Blood
Scale
Mini
Elution Volume
50 to 200 μL
Final Product Type
gDNA
For Use With (Application)
PCR, Southern blotting, sequencing, nucleic acid labeling, hybridization
High-throughput Compatibility
Not High-throughput Compatible (Manual)
Quantity
250 Preps
Shipping Condition
Room Temperature
Test Time
20 min. (After sample homogenization)
Yield
≤65 μg
Starting Material Amount
Bone Marrow: ≤200 μL
Buccal Swabs: 1
Buffy Coat: 200 μL
Dried Blood Spots: varies
Urine: ≤4.5 mL
Whole Blood: ≤500 μL

Contents & Storage

• 5 x 1.2 mL Proteinase K Solution; store at room temperature until opened, –20°C after opening
• 120 mL Lysis Solution; room temperature
• 40 mL Wash Buffer I (concentrated); room temperature
• 40 mL Wash Buffer II (concentrated); room temperature
• 150 mL Elution Buffer; room temperature
• 250 GeneJET Purification Columns with Collection Tubes; room temperature
• 250 Collection Tubes; room temperature

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