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Thermo Scientific™

GeneJET Plant RNA Purification Kit

Catalog number: K0801
Thermo Scientific™

GeneJET Plant RNA Purification Kit

Catalog number: K0801
Catalog Number
K0801
Unit Size
50 preps
Price (USD)
Price: 269.00
Online price: 234.65
Your price:
Availability
-
Quantity
Catalog Number
Unit Size
250 preps
Price (USD)
Price: 1,062.00
Online price: 922.65
Your price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
K080150 preps
Price: 269.00
Online price: 234.65
Your price:
-
K0802250 preps
Price: 1,062.00
Online price: 922.65
Your price:
-
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Thermo Scientific GeneJET Plant RNA Purification Mini Kit is designed for rapid and efficient purification of high quality total RNA from wide variety of plant species and tissue types.

The kit utilizes silica-based membrane technology in the form of a convenient spin column, eliminating the need for expensive resins, toxic phenol-chloroform extractions, or time-consuming alcohol precipitation. The standard procedure takes less than 20 minutes following cell lysis. Purified high quality RNA can be used in a wide range of downstream applications, such as RT-PCR, RT-qPCR, Northern blotting and other RNA-based analysis. RNA yields vary between different species, tissues, and age of tissue sample. See Table 1 for typical total RNA yields from various sources.

Highlights
Fast—procedure completed in 20 min following cell lysis
Convinient—protocol does not include clogging-prone lysate filtration step
High yield—up to 65 μg of high purity and high integrity RNA from 50 mg plant sample
Compatible with wide variety of samples (leaves, roots, sprouts and other parts of various plants)

Applications
Fast extraction of high purity RNA suitable for all conventional molecular biology procedures, including:
• RT-PCR
• RT-qPCR
• Northern blotting
• Nuclease protection assay

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Isolation Technology
Silica Spin Column
Purification Time
20 min. (After sample homogenization)
Elution Volume
50 μL
Sample Type
Plants
Final Product Type
Total RNA
High-throughput Compatibility
Not High-throughput Compatible (Manual)
For Use With (Application)
RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
Quantity
50 Preps
Scale
Mini
Shipping Condition
Room Temperature
Starting Material Amount
≤100 mg
Yield
Up to 65 μg

Contents & Storage

• 40 mL Plant RNA Lysis Solution; store at room temperature
• 40 mL Wash Buffer WB 1 (concentrated); room temperature
• 30 mL Wash Buffer 2 (concentrated); room temperature
• 30 mL Water, Nuclease-free; room temperature
• 50 GeneJET RNA Spin Columns with Collection Tubes; room temperature
• 50 Collection Tubes (2 mL); room temperature
• 50 Collection Tubes (1.5 mL); room temperature

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