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Thermo Scientific™

GeneJET Endo-Free Plasmid Maxiprep Kit

Catalog number: K0861
Thermo Scientific™

GeneJET Endo-Free Plasmid Maxiprep Kit

Catalog number: K0861
Catalog Number
K0861
Unit Size
10 preps
Price (USD)
Price: 294.00
Online price: 230.65
Your price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
K086110 preps
Price: 294.00
Online price: 230.65
Your price:
-
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Thermo Scientific GeneJET Endo-Free Plasmid Maxiprep Kit is a fast, convenient, and efficient system for the large scale purification of endotoxin-free plasmid DNA. Endotoxins are major contaminants found in plasmid DNA preparations. For transfection of sensitive cell lines endotoxins are a major problem. The same applies to in vivo transfection as endotoxins can elicit strong immune responses. Thermo Scientific GeneJET Endo-Free Plasmid Maxiprep Kit is designed to reduce endotoxin level to <0.1 EU/µg of plasmid DNA. The kit utilizes a silica-based membrane technology in the form of convenient spin columns.

Each preparation recovers up to 1000 µg of high copy plasmid DNA from 350 mL of recombinant E. coli cultures that can be used in a wide variety of molecular biology procedures such as transfection of sensitive cells (e.g. Huh-7), in vivo transfection, transformation, restriction endonuclease digestion, automated sequencing, and in vitro transcription.

Highlights

Pure—free of endotoxins plasmid DNA suitable for transfection of sensitive cells
Efficient—plasmid recovery resulting in high DNA yields
Fast—isolation of high quality plasmid DNA in less than 60 minutes
Convenient—spin column format, centrifuge or vacuum protocol

Applications

• Transfection of sensitive cell lines
• in vivo transfection
• Automated sequencing
• PCR
• Transformation
• in vitro transcription

Includes
• Resuspension Solution
• Lysis Solution
• Neutralization Solution
• Endotoxin Binding Reagent
• Wash Solution I (conc.)
• Wash Solution II (conc.)
• RNase A Solution
• Elution Buffer
• GeneJET Endo-Free Maxi Purification Columns & Collection Tubes (50 mL, blue-capped)
• GeneJET Endo-Free Maxi Filtration Columns & Collection Tubes (50 mL, white-capped)
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Concentration
400 ng/μL
Column Type
Spin Column
Format
Spin Column
Isolation Technology
Silica Column
Sample Type
Bacteria
Scale
Maxi
For Use With (Application)
Real-Time Quantitative PCR (qPCR), Transfection, Cloning, Sequencing, In Vitro Transcription, Transformation, Nucleic Acid Labeling, PCR
High-throughput Compatibility
Not High-throughput Compatible (Manual)
No. of Reactions
10 Preps
Prep Scale
> 200 μg (Large-Scale) Plasmid DNA
Product Line
GeneJET
Product Type
Endo-Free Plasmid MaxiPrep Kit
Quantity
10 Preps
Target
Plasmid DNA
Test Time
60 min.

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