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Thermo Scientific™

GeneJET Stabilized and Fresh Whole Blood RNA Kit

Catalog number: K0871
Thermo Scientific™

GeneJET Stabilized and Fresh Whole Blood RNA Kit

Catalog number: K0871
Catalog Number
K0871
Unit Size
50 preps
Price (USD)
Price: 345.00
Your price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
K087150 preps
Price: 345.00
Your price:
-
Product Overview
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Thermo Scientific GeneJET™ Stabilized and Fresh Whole Blood RNA Kit is designed for rapid and efficient purification of high quality total RNA from various whole blood samples: fresh, frozen, or human blood samples stabilized with commercial stabilizers such as Tempus™ and PAXgene™.

The kit utilizes silica-based membrane technology in the form of a convenient spin column, eliminating the need for expensive resins, toxic phenol-chloroform extractions, or time-consuming alcohol precipitation. The kit includes a DNase l for the enzymatic removal of genomic DNA. RNA can be isolated from whole blood in less than 30 minutes (including cell lysis and DNase l treatment).

Highlights

Versatile—compatible with fresh and frozen blood, buffy coat, as well as blood collected in PAXgene™and Tempus™ Tubes
Convenient—specially optimized Lysis Buffer is suitable for RNA stabilization up to 48 h
Efficient—up to 4 μg of total RNA from 500 μL of healthy human blood
Pure—isolates intact total RNA free of any RT-qPCR inhibitors

Applications

Isolated total RNA can be used in:
• RT-PCR
• RT-qPCR
• Northern Blotting

Includes
• Lysis Buffer (R2841)
• Wash Buffer 1 (conc.)(R2851)
• Wash Buffer 2 (conc.)(R2861)
• DNase l (lyophilized)
• DNase I Reconstitution Buffer
• 2x DNase I Buffer
• Manganese Chloride Solution
• Water, nuclease-free
• GeneJET RNA Purification Columns pre-assembled with Collection Tubes
• Collection Tubes, 2 mL
• Collection Tubes, 1.5 mL

Related Products
Lysis Buffer for GeneJET Blood RNA Kit
Wash Buffer 1 for GeneJET Blood RNA Kit (concentrated)
Wash Buffer 2 for GeneJET Blood RNA Kit (concentrated)
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Concentration
2X
Format
Spin Column
Isolation Technology
Spin Column
Purification Time
30 min.
Sample Type
Blood
High-throughput Compatibility
Not High-throughput Compatible (Manual)
For Use With (Application)
Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Nuclease Protection Assays, Nucleic Acid Labeling, Northern Blotting, cDNA Library Construction
No. of Reactions
50 Preps
Product Line
GeneJET
Target
Total RNA

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