Thermo Scientific™

Luminaris Probe qPCR Master Mix, high ROX

Catalog number: K0931
Thermo Scientific™

Luminaris Probe qPCR Master Mix, high ROX

Catalog number: K0931
Catalog Number
K0931
Unit Size
250 reactions
Price (USD)
231.00
Your Price:
Availability
***
Quantity
Catalog Number
Unit Size
500 reactions
Price (USD)
417.00
Your Price:
Availability
***
Quantity
Catalog Number
Unit Size
1250 reactions
Price (USD)
962.00
Your Price:
Availability
***
Quantity
Catalog Number
Unit Size
5,000 reactions
Price (USD)
2,700.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
K0931250 reactions
231.00
Your Price:
***
K0932500 reactions
417.00
Your Price:
***
K09331250 reactions
962.00
Your Price:
***
K09345,000 reactions
2,700.00
Your Price:
***
Product Overview
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Additional Information
Recommendations
Recommendations
Thermo Scientific Luminaris Color Probe and Luminaris Probe qPCR Master Mixes are universal ready-to-use solutions optimized for qPCR and two-step RT-qPCR. The master mixes include Thermo Scientific Hot Start Taq DNA polymerase, uracil-DNA glycosylase (UDG) and dNTPs in an optimized PCR buffer. Only the template, primers and probe need to be added.

The Hot Start Taq DNA polymerase, in combination with an optimized buffer, ensures PCR specificity and sensitivity. dUTP and UDG are included in the mix for carry-over contamination control (see Reference 1).

The use of Luminaris Color Probe and Luminaris Probe qPCR Master Mixes in qPCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates. Individual formulations of Luminaris Color Probe and Luminaris Probe qPCR Master Mixes with ROX at different concentrations as passive reference dye are available for most qPCR platforms (see Real-time PCR instrument compatibility chart).

Highlights

• UDG in the master mix to prevent carry-over contamination• Specificity—Hot Start Taq DNA Polymerase and the optimized buffer reduce primer dimers
• Sensitivitity—single copy detection
• Wide linear range—accurate quantification across 9 orders of magnitude
• Reproducibility and convenience— ready-to-use 2X master mix
• Equal performance of blue and colorless master mixes
• Versions with premixed ROX for different real-time platforms
• Standard cycling protocols

Applications

• Gene expression analysis
• siRNA validation
• Genotyping
• Pathogen detection

Includes

Luminaris Probe High ROX qPCR Master Mix (2X) includes Hot Start Taq Polymerase, UDG and dNTPs (also dUTP) in an optimized PCR buffer. The master mix contains high concentration of ROX passive reference dye for specific real-time platforms (see Real-Time PCR instrument compatibility chart) and is supplied with nuclease-free water.

Specifications

Concentration
2 X
Detection Method
Primer-Probe Detection
Fidelity (vs. Taq)
2 X
GC-Rich PCR Performance
High
No. of Reactions
250 Reactions
PCR Method
qPCR
Polymerase
Taq DNA Polymerase
Reaction Speed
Standard
Sample Type
DNA, dsDNA
For Use With (Application)
qPCR, Two-Step RT-qPCR
For Use With (Equipment)
7000 System, 7300 System, 7700 System, 7900HT System, StepOnePlus™ System, StepOne™ System
No. of Tests
250 Reactions
Product Line
Luminaris
Product Type
qPCR Master Mix
Sufficient For
250 Reactions

Contents & Storage

Contains:
• 2 x 1.25 mL Luminaris Probe High ROX qPCR Master Mix (2X) containing Hot Start Taq DNA Polymerase, UDG and dNTPs (with dUTP) in an optimized PCR buffer; sufficient for 250 x 20 µL reactions. The master mix contains high concentration of ROX passive reference dye for specific real-time platforms.
• 2 x 1.25 mL Nuclease-Free Water

Store at -20°C.

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