Luminaris Probe qPCR Master Mix, high ROX

Catalog number:  K0934

Thermo Scientific™  Related applications: Real Time PCR (qPCR)

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Description

Thermo Scientific Luminaris Color Probe and Luminaris Probe qPCR Master Mixes are universal ready-to-use solutions optimized for qPCR and two-step RT-qPCR. The master mixes include Thermo Scientific Hot Start Taq DNA polymerase, uracil-DNA glycosylase (UDG) and dNTPs in an optimized PCR buffer. Only the template, primers and probe need to be added.

The Hot Start Taq DNA polymerase, in combination with an optimized buffer, ensures PCR specificity and sensitivity. dUTP and UDG are included in the mix for carry-over contamination control (see Reference 1).

The use of Luminaris Color Probe and Luminaris Probe qPCR Master Mixes in qPCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates. Individual formulations of Luminaris Color Probe and Luminaris Probe qPCR Master Mixes with ROX at different concentrations as passive reference dye are available for most qPCR platforms (see Real-time PCR instrument compatibility chart).

Highlights

• UDG in the master mix to prevent carry-over contamination• Specificity—Hot Start Taq DNA Polymerase and the optimized buffer reduce primer dimers
• Sensitivitity—single copy detection
• Wide linear range—accurate quantification across 9 orders of magnitude
• Reproducibility and convenience— ready-to-use 2X master mix
• Equal performance of blue and colorless master mixes
• Versions with premixed ROX for different real-time platforms
• Standard cycling protocols

Applications

• Gene expression analysis
• siRNA validation
• Genotyping
• Pathogen detection

Includes

Luminaris Probe High ROX qPCR Master Mix (2X) includes Hot Start Taq Polymerase, UDG and dNTPs (also dUTP) in an optimized PCR buffer. The master mix contains high concentration of ROX passive reference dye for specific real-time platforms (see Real-Time PCR instrument compatibility chart) and is supplied with nuclease-free water.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Detection Method: Primer-Probe Detection
For Use With (Equipment): 7000 System, 7300 System, 7700 System, 7900HT System, StepOne™ System, StepOnePlus™ System
Reaction Speed: Standard
PCR Method: qPCR
Sample Type (General): DNA, dsDNA
Number of Reactions: 5,000 Reactions
Polymerase: Taq DNA Polymerase
Product Line: Luminaris
Product Size: 5,000 reactions

Contents & storage

Contains:
• 4 x 12.5 mL Luminaris Probe High ROX qPCR Master Mix (2X) containing Hot Start Taq DNA Polymerase, UDG and dNTPs (with dUTP) in an optimized PCR buffer; sufficient for 5000 x 20 µL reactions. The master mix contains high concentration of ROX passive reference dye for specific real-time platforms.
• 2 x 30 mL Nuclease-Free Water

Store at -20°C.

Documents

Manuals & protocols