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Thermo Scientific™
Luminaris Probe qPCR Master Mix
Alternative Product: Try TaqMan Fast Advanced Master Mix, our highest-performance, probe-based master mix. With TaqMan Fast Advanced Master Mix, we’veMás información
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| Número de catálogo | N.º de reacciones |
|---|---|
| K0951 | 250 reacciones |
| K0952 | 500 reacciones |
| K0953 | 1250 reacciones |
| K0954 | 5000 reacciones |
Número de catálogo K0951
Precio (MXN)
-
N.º de reacciones:
250 reacciones
Alternative Product: Try TaqMan Fast Advanced Master Mix, our highest-performance, probe-based master mix. With TaqMan Fast Advanced Master Mix, we’ve taken the best of Luminaris Probe qPCR Master Mix and added additional capabilities for your gene expression analysis.
Thermo Scientific Luminaris Color Probe and Luminaris Probe qPCR Master Mixes are universal ready-to-use solutions optimized for qPCR and two-step RT-qPCR. The master mixes include Thermo Scientific Hot Start Taq DNA polymerase, uracil-DNA glycosylase (UDG) and dNTPs in an optimized PCR buffer. Only the template, primers and probe need to be added.
The Hot Start Taq DNA polymerase, in combination with an optimized buffer, ensures PCR specificity and sensitivity. dUTP and UDG are included in the mix for carry-over contamination control (see Reference 1).
The use of Luminaris Color Probe and Luminaris Probe qPCR Master Mixes in qPCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates. Individual formulations of Luminaris Color Probe and Luminaris Probe qPCR Master Mixes with ROX at different concentrations as passive reference dye are available for most qPCR platforms (see Real-time PCR instrument compatibility chart).
Highlights
• UDG in the master mix to prevent carry-over contamination
• Specificity—Hot Start Taq DNA Polymerase and the optimized buffer reduce primer dimersz
• Sensitivitity—single copy detection
• Wide linear range—accurate quantification across 9 orders of magnitude
• Reproducibility and convenience—ready-to-use 2X master mix
• Equal performance of blue and colorless master mixes
• Versions with premixed ROX for different real-time platforms
• Standard cycling protocols
Applications
• Gene expression analysis
• siRNA validation
• Genotyping
• Pathogen detection
Includes
Luminaris Probe qPCR Master Mix (2X) includes Hot Start Taq Polymerase, UDG and dNTPs (also dUTP) in an optimized PCR buffer; supplied with nuclease-free water.
Thermo Scientific Luminaris Color Probe and Luminaris Probe qPCR Master Mixes are universal ready-to-use solutions optimized for qPCR and two-step RT-qPCR. The master mixes include Thermo Scientific Hot Start Taq DNA polymerase, uracil-DNA glycosylase (UDG) and dNTPs in an optimized PCR buffer. Only the template, primers and probe need to be added.
The Hot Start Taq DNA polymerase, in combination with an optimized buffer, ensures PCR specificity and sensitivity. dUTP and UDG are included in the mix for carry-over contamination control (see Reference 1).
The use of Luminaris Color Probe and Luminaris Probe qPCR Master Mixes in qPCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates. Individual formulations of Luminaris Color Probe and Luminaris Probe qPCR Master Mixes with ROX at different concentrations as passive reference dye are available for most qPCR platforms (see Real-time PCR instrument compatibility chart).
Highlights
• UDG in the master mix to prevent carry-over contamination
• Specificity—Hot Start Taq DNA Polymerase and the optimized buffer reduce primer dimersz
• Sensitivitity—single copy detection
• Wide linear range—accurate quantification across 9 orders of magnitude
• Reproducibility and convenience—ready-to-use 2X master mix
• Equal performance of blue and colorless master mixes
• Versions with premixed ROX for different real-time platforms
• Standard cycling protocols
Applications
• Gene expression analysis
• siRNA validation
• Genotyping
• Pathogen detection
Includes
Luminaris Probe qPCR Master Mix (2X) includes Hot Start Taq Polymerase, UDG and dNTPs (also dUTP) in an optimized PCR buffer; supplied with nuclease-free water.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Fidelidad (frente a Taq)2 X
Para utilizar con (equipo)BioRad CFX96™, BioRad iCycler iQ, BioRad iQ5, BioRad MyiQ, MJ Chromo4, Eppendorf MasterCycler RealPlex, Corbett RotorGene, Bio-Rad CFX384 Touch™, Roche LightCycler 480, Cepheid SmartCycler, MJ Opticon
N.º de reacciones250 reacciones
PolimerasaADN polimerasa Taq
Línea de productosLuminaris
Tipo de productoqPCR Master Mix
Cantidad250 reactions
Tipo de muestraADN, ADNbc
Suficiente para250 reacciones
Concentración2X
Método de detecciónSonda de cebado
Para utilizar con (aplicación)Gene Expression
GC-Rich PCR PerformanceHigh
Método de PCRqPCR
Velocidad de reacciónEstándar
Unit SizeEach
Contenido y almacenamiento
Contains:
• 2 x 1.25 mL Luminaris Probe qPCR Master Mix (2X) containing Hot Start Taq DNA Polymerase, UDG and dNTPs (with dUTP) in an optimized PCR buffer; sufficient for 250 x 20 μL reactions
• 2 x 1.25 mL Nuclease-Free Water
Store at -20°C.
• 2 x 1.25 mL Luminaris Probe qPCR Master Mix (2X) containing Hot Start Taq DNA Polymerase, UDG and dNTPs (with dUTP) in an optimized PCR buffer; sufficient for 250 x 20 μL reactions
• 2 x 1.25 mL Nuclease-Free Water
Store at -20°C.