Error:Error:As of December 31, 2021, this product will be discontinued and will remain in stock until sold out. We recommend PowerTrack™ SYBR Green Master Mix—our newest and best performing SYBR green mix that features a two-color tracking dye system to reduce pipetting errors.
Thermo Scientific™

Luminaris HiGreen qPCR Master Mix, high ROX

Catalog number: K0961
Thermo Scientific™

Luminaris HiGreen qPCR Master Mix, high ROX

Catalog number: K0961
Catalog Number
K0961
Unit Size
250 reactions
Price (USD)
225.00
Your Price:
Availability
***
Quantity
Catalog Number
Unit Size
1250 reactions
Price (USD)
952.00
Your Price:
Availability
***
Quantity
Catalog Number
Unit Size
500 reactions
Price (USD)
417.00
Your Price:
Availability
***
Quantity
Catalog Number
K0964
also known as 553955395
Unit Size
5,000 reactions
Price (USD)
2,640.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
K0961250 reactions
225.00
Your Price:
***
K09631250 reactions
952.00
Your Price:
***
K0962500 reactions
417.00
Your Price:
***
K0964
also known as 553955395
5,000 reactions
2,640.00
Your Price:
***
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Recommendations
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Alternative Product: Try PowerUp SYBR Green Master Mix, our newest, high-performance, SYBR dye-based master mix for superior performance at a very competitive price. With PowerUp SYBR Green Master Mix, we’ve taken the best of Luminaris HiGreen qPCR Master Mix and added additional capabilities for your gene expression analysis.

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Thermo Scientific Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes are universal ready-to-use solutions optimized for qPCR and two-step RT-qPCR. The master mixes include Thermo Scientific Hot Start Taq DNA polymerase, uracil-DNA glycosylase (UDG) and dNTPs in an optimized PCR buffer. Only the template and primers need to be added. The SYBR Green I intercalating dye allows for DNA detection and analysis without using sequence-specific probes.

The Hot Start Taq DNA polymerase in combination with an optimized buffer ensures PCR specificity and sensitivity. dUTP and UDG are included in the mix for carry-over contamination control (see Reference 1).

The use of Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes in qPCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates. Individual formulations of Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes with ROX at different concentrations or fluorescein as passive reference dyes are available for most qPCR platforms (see Real-time PCR instrument compatibility chart).

Highlights

• UDG in the master mix to prevent carry-over contamination
• Equal performance of blue and colorless master mixes
• Versions with premixed ROX or fluorescein for different real-time platforms
• Standard cycling protocols
• Excellent qPCR performance:
Specificity: Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers
Sensitivity: ingle copy detection
Wide linear range: accurate quantification across 9 orders of magnitude
Reproducibility and convenience: ready-to-use 2X master mix


Applications

• Gene expression analysis
• siRNA validation
• Genotyping
• Pathogen detection

Includes

Luminaris HiGreen High ROX qPCR Master Mix (2X) includes Hot Start Taq DNA Polymerase, UDG and dNTPs (also dUTP) in an optimized PCR buffer. The master mix contains high concentration of ROX passive reference dye for specific real-time platforms (see Real-Time PCR instrument compatibility chart) and is supplied with nuclease-free water.

Specifications

Concentration
2 X
Detection Method
SYBR
Fidelity (vs. Taq)
2 X
GC-Rich PCR Performance
High
No. of Reactions
250 Reactions
PCR Method
qPCR
Polymerase
Taq DNA Polymerase
Reaction Speed
Standard
Sample Type
DNA, dsDNA
For Use With (Application)
qPCR, Two-Step RT-qPCR
For Use With (Equipment)
7000 System, 7300 System, 7700 System, 7900HT System, StepOnePlus™ System, StepOne™ System
No. of Tests
250 Reactions
Product Line
Luminaris
Product Type
qPCR Master Mix
Sufficient For
250 Reactions

Contents & Storage

Contains:
• 2 x 1.25 mL Luminaris HiGreen High ROX qPCR Master Mix (2X) containing Hot Start Taq DNA Polymerase, UDG, dNTPs (with dUTP) and SYBR Green I in an optimized PCR buffer; sufficient for 250 x 20 µL reactions. The master mix contains high concentration of ROX passive reference dye for specific real-time platforms.
• 2 x 1.25 mL Nuclease-Free Water

Store at -20°C.

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