Thermo Scientific™

Luminaris HiGreen qPCR Master Mix

Catalog number: K0993
Thermo Scientific™

Luminaris HiGreen qPCR Master Mix

Catalog number: K0993
Catalog Number
K0993
Unit Size
1250 reactions
Price (USD)
874.00
Your Price:
Availability
***
Quantity
Catalog Number
Unit Size
500 reactions
Price (USD)
384.00
Your Price:
Availability
***
Quantity
Catalog Number
Unit Size
250 reactions
Price (USD)
210.00
Your Price:
Availability
***
Quantity
Catalog Number
Unit Size
5,000 reactions
Price (USD)
2,455.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
K09931250 reactions
874.00
Your Price:
***
K0992500 reactions
384.00
Your Price:
***
K0991250 reactions
210.00
Your Price:
***
K09945,000 reactions
2,455.00
Your Price:
***
Product Overview
Figures
Videos
Documents
FAQ
Citations & References
Additional Information
Recommendations
**********************
Alternative Product: Try PowerUp SYBR Green Master Mix, our newest, high-performance, SYBR dye-based master mix for superior performance at a very competitive price. With PowerUp SYBR Green Master Mix, we’ve taken the best of Luminaris HiGreen qPCR Master Mix and added additional capabilities for your gene expression analysis.

**********************
Thermo Scientific Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes are universal ready-to-use solutions optimized for qPCR and two-step RT-qPCR. The master mixes include Thermo Scientific Hot Start Taq DNA polymerase, uracil-DNA glycosylase (UDG) and dNTPs in an optimized PCR buffer. Only the template and primers need to be added. The SYBR Green I intercalating dye allows for DNA detection and analysis without using sequence-specific probes.

The Hot Start Taq DNA polymerase in combination with an optimized buffer ensures PCR specificity and sensitivity. dUTP and UDG are included in the mix for carry-over contamination control (see Reference 1).

The use of Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes in qPCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates. Individual formulations of Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes with ROX at different concentrations or fluorescein as passive reference dyes are available for most qPCR platforms (see Real-time PCR instrument compatibility chart).

Highlights:

• UDG in the master mix to prevent carry-over contamination
• Equal performance of blue and colorless master mixes
• Versions with premixed ROX or fluorescein for different real-time platforms
• Standard cycling protocols
• Excellent qPCR performance:
Specificity: Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers
Sensitivity: single copy detection
Wide linear range: accurate quantification across 9 orders of magnitude
Reproducibility and convenience: ready-to-use 2X master mix

Applications:

• Gene expression analysis
• siRNA validation
• Genotyping
• Pathogen detection

Includes:

Luminaris HiGreen qPCR Master Mix (2X) includes Hot Start Taq DNA Polymerase, UDG and dNTPs (also dUTP) in an optimized PCR buffer; supplied with nuclease-free water.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

For Use With (Application)
qPCR, Two-Step RT-qPCR
For Use With (Equipment)
BioRad CFX96™, MJ Chromo4, MJ Opticon, Eppendorf MasterCycler RealPlex, Bio-Rad CFX384 Touch™, Roche LightCycler 480, Cepheid SmartCycler, Corbett RotorGene
No. of Tests
1250 Reactions
Product Line
Luminaris
Product Type
qPCR Master Mix
Sufficient For
250 Reactions
Concentration
2 X
Detection Method
SYBR
Fidelity (vs. Taq)
2 X
GC-Rich PCR Performance
High
No. of Reactions
1,250 Reactions
PCR Method
qPCR
Polymerase
Taq DNA Polymerase
Reaction Speed
Standard
Sample Type
DNA, dsDNA

Contents & Storage

Contains:
• 10 x 1.25 mL Luminaris HiGreen qPCR Master Mix (2X) containing Hot Start Taq DNA Polymerase, UDG, dNTPs (with dUTP) and SYBR Green I in an optimized PCR buffer; sufficient for 1250 x 20 µL reactions
• 10 x 1.25 mL Nuclease-Free Water

Store at -20°C.

Figures

Frequently asked questions (FAQs)

Citations & References