CCF2-AM, our LiveBLAzer™ substrate, is a lipophilic, esterified form of the CCF2 substrate which allows it to readily enter cells. Upon entry, cleavage by endogenous cytoplasmic esterases rapidly converts CCF2-AM into its negatively charged form, CCF2 or CCF4, which is retained in the cytosol. CCF2 is a Fluorescence Resonance Energy Transfer (FRET) substrate which consists of a cephalosporin core linking 7-hydroxycoumarin to fluorescein. This product is to be used as a FRET-based substrate for β-lactamase as a sensitive reporter of mammalian gene expression in the GeneBLAzer® technology.
For Research Use Only. Not for use in diagnostic procedures.
For Use With (Equipment)
Flow Cytometer, Microplate Reader
Contents & Storage
Store in freezer (-5 to -30°C).
Figure 7 - Example of an assay panel developed with GenomeScreen™ assay to decouple closely related pathways (here, INF-α and INF-γ)
Figure 1 - Using GeneBLAzer™ Technology to report on diverse signal transduction pathways