CCF4-AM is also available in a lower priced smaller pack size as a component of the LiveBLAzer™ FRET-B/G Loading Kit with CCF4-AM.

CCF4-AM

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Description

CCF4-AM, our LiveBLAzer™ substrate, is a lipophilic, esterified form of the CCF4 substrate which allows it to readily enter cells. Upon entry, cleavage by endogenous cytoplasmic esterases rapidly converts CCF4-AM into its negatively charged form, CCF4, which is retained in the cytosol. CCF4 is a Fluorescence Resonance Energy Transfer (FRET) substrate which consists of a cephalosporin core linking 7-hydroxycoumarin to fluorescein. This product is to be used as a FRET-based substrate for β-lactamase as a sensitive reporter of mammalian gene expression in the GeneBLAzer® technology.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Cell Permeability: Cell-Permeant
Detection Method: Fluorescent
For Use With (Equipment): Flow Cytometer, Microplate Reader
Product Line: LiveBLAzer™
Product Size: 20 mg
Substrate: CCF4-AM
Substrate Properties: Chemical Substrate
Substrate Type: Beta-Lactamase Substrate
Shipping Condition: Room Temperature

Contents & storage

Store in freezer (-5 to -30°C).

Documents

Manuals & protocols