CCF4-AM, our LiveBLAzer™ substrate, is a lipophilic, esterified form of the CCF4 substrate which allows it to readily enter cells. Upon entry, cleavage by endogenous cytoplasmic esterases rapidly converts CCF4-AM into its negatively charged form, CCF4, which is retained in the cytosol. CCF4 is a Fluorescence Resonance Energy Transfer (FRET) substrate which consists of a cephalosporin core linking 7-hydroxycoumarin to fluorescein. This product is to be used as a FRET-based substrate for β-lactamase as a sensitive reporter of mammalian gene expression in the GeneBLAzer® technology.
For Research Use Only. Not for use in diagnostic procedures.
For Use With (Equipment)
Flow Cytometer, Microplate Reader
Contents & Storage
Store in freezer (-5 to -30°C).
Figure 4 - Beta-lactamase expression following treatment with known agonists and antagonists in the GeneBLAzer® M1 NFAT-bla Jurkat Cell Line
Figure 6 - Experimental outline of GenomeScreen™ assay
Figure 7 - Example of an assay panel developed with GenomeScreen™ assay to decouple closely related pathways (here, INF-α and INF-γ)
Figure 1 - Using GeneBLAzer™ Technology to report on diverse signal transduction pathways